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Regulation of TLR8-activated gene expression in human neutrophils [RNA-seq HL-60]

ABSTRACT: The transcription factors that regulate gene expression in stimulated neutrophils are poorly characterized. Herein, we found that the genomic distribution of the myeloid lineage determining transcription factors PU.1 and C/EBPβ, as well as that of the histone modification H3K27Ac, which marks active chromatin, significantly change in human neutrophils treated with R848, a ligand of TLR8.

ORGANISM(S): Homo sapiens

PROVIDER: GSE119393 | GEO | 2021/05/07

REPOSITORIES: GEO

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Regulation of TLR8-activated gene expression in human neutrophils [RNA-seq neutrophils & monocytes]

Project description:The transcription factors that regulate gene expression in stimulated neutrophils are poorly characterized. Herein, we found that the genomic distribution of the myeloid lineage determining transcription factors PU.1 and C/EBPβ, as well as that of the histone modification H3K27Ac, which marks active chromatin, significantly change in human neutrophils treated with R848, a ligand of TLR8.

2021-05-07 | GSE119394 | GEO

Regulation of TLR8-activated gene expression in human neutrophils [ChIP-seq]

2021-05-07 | GSE119392 | GEO

SARS-CoV-2-associated ssRNAs activate human neutrophils in a TLR8-dependent fashion

Project description:COVID-19 disease is characterized by a dysregulation of the innate arm of the immune system. However, the mechanisms whereby innate immune cells, including neutrophils, become activated in patients are not completely understood. Recently, we showed that a GU-rich RNA sequence from SARS-CoV-2 genome (i.e., SCV2-RNA2) activates dendritic cells. To clarify whether human neutrophils may also represent targets of SCV2-RNA2, neutrophils were treated with either SCV2-RNA2 or, as control, R848 (a TLR7/8 ligand), and then analysed for several functional assays and also subjected to RNA-seq experiments. Results highlight a remarkable response of neutrophils to SCV2-RNA2 in terms of TNFa, IL1ra, CXCL8 production, apoptosis delay, modulation of CD11b and CD62L expression and release of neutrophil extracellular traps. By RNA-seq experiments, we observed that SCV2-RNA2 promotes a transcriptional reprogramming of neutrophils, characterized by the induction of thousands of proinflammatory genes, similarly to that promoted by R848. Furthermore, by using CU-CPT9a, a TLR8-specific inhibitor, we found that SCV2-RNA2 stimulates neutrophils exclusively via TLR8-dependent pathways. In sum, our study proves that single-strand RNAs from the SARS-CoV-2 genome potently activate human neutrophils via TLR8, thus uncovering a potential mechanism whereby neutrophils may contribute to the pathogenesis of severe COVID-19 disease.

2023-01-06 | GSE214247 | GEO

Regulation of TLR8-activated gene expression in human neutrophils [RNA-seq HL-60]

Project description:Regulation of TLR8-activated gene expression in human neutrophils [RNA-seq HL-60]

| PRJNA489113 | ENA

Regulation of TLR8-activated gene expression in human neutrophils [RNA-seq neutrophils & monocytes]

Project description:Regulation of TLR8-activated gene expression in human neutrophils [RNA-seq neutrophils & monocytes]

| PRJNA489111 | ENA

Regulation of TLR8-activated gene expression in human neutrophils

Project description:Regulation of TLR8-activated gene expression in human neutrophils

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Regulation of TLR8-activated gene expression in human neutrophils [ChIP-seq]

Project description:Regulation of TLR8-activated gene expression in human neutrophils [ChIP-seq]

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Transcriptome sequencing in HL-60 induced neutrophils between the stimulation of AGEs and high-glucose

Project description:We report the high-throughput sequencing results in mammalian cell lines. Neutrophils were induced by HL-60 with 1umol/L ATRA(all-trans retinoid acid ) stimulation for 7days. Neutrophils were then stimulated with 0.05ug/mL AGEs，30mmol/L D-glucose, AGEs+D-glucose and 0.05ug/mL BSA (control) for 24-hours, then mRNA profiles were extracted and tested using Illumina Hiseq 4000. This study provides a prospective work for the influence of AGEs and high-glucose in human neutrophils.

2022-12-09 | GSE163687 | GEO

Genome-wide C/EBPβ traget genes in C/EBPβ-overexpressed HL-60 cells.

Project description:To characterize genome-wide C/EBPβ traget genes in C/EBPβ-overexpressed HL-60 cells by ChIP-seq.

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Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils [HL60]

Project description:To investigate the changes in gene expression during the process of differentiation into neutrophils, microarray analysis was performed. HL-60 cells were cultured with all-trans retinoic acid (ATRA) for 4 days. The cells were collected at four different time points and total RNA was prepared for use as a sample for microarray analysis.

2022-12-28 | GSE200823 | GEO

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