Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA expression of stretched versus unstretched primary murine sinusoidal endothelial cells.

ABSTRACT: Primary murine sinusoidal endothelial cells were subjected to cyclic stretch and their gene expression profiles assessed by RNA-sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE119547 | GEO | 2018/09/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA expression of stretched versus unstretched primary murine sinusoidal endothelial cells.

Project description:RNA expression of stretched versus unstretched primary murine sinusoidal endothelial cells.

| PRJNA489495 | ENA

Expression profiling of mechanical stretched interfollicular epidermal stem cells (IFESCs)

Project description:The renewing human epidermis constantly senses and adapts to a wide range of mechanical cues that are ubiquitous throughout life. The mechanisms of how mechanical forces are responded by interfollicular epidermal stem cells (IFESCs) and are transmitted directly into nucleus to modify gene expression remain incompletely defined. In vitro, human IFESCs were cultured on the collagen I coated silicon rubber membrane and then subjected to the mechanical stretched. Cyclic mechanical tension at 0.5 Hz sinusoidal curve at 10% elongation was applied using an FX-5000T™ Flexercell® Tension Plus™ unit (Flexcell International Corporation). In mechanical unloading groups, cells were cultured on the same plates in the same incubator with the mechanical stretched groups but not subjected to stretch. Combining genome-wide microarray and functional analyses, we made transcriptome analysis of samples from the mechanical unstretched or stretched isolated human IFESCs.

2018-07-26 | GSE71391 | GEO

Expression data from cyclically stretched engineered neonatal rat ventricuar myocyte (NRVM) tissues

2013-01-30 | GSE43846 | GEO

Aging-associated transcriptome changes in liver sinusoidal endothelial cells.

Project description:Aging-associated transcriptome changes in liver sinusoidal endothelial cells. RNA was prepared from liver sinusoidal endothelial cells of the male 8 weeks and 24 months old C57BL/6J mice.RNA samples were then subjected to RNA-sequencing and gene expression profiling analysis.

2022-10-29 | GSE216592 | GEO

Expression data from cyclically stretched engineered neonatal rat ventricuar myocyte (NRVM) tissues

Project description:Mechanical overload in the heart induces pathological remodeling that typcially leads to heart failure. We sought to build an in vitro model of heart failure by applying cyclic stretch to engineered isotropic (iso) and anisotropic (aniso) NRVM tissues. We used micoarrays to determine the effects of longitudinal and transvserse cyclic stretch on gene expression in engineered NRVM cardiac tissues. We found that cyclic stretch induced up-regulation of several known indicators of heart faliure, independent of the direction of stretch. NRVMs were seeded on silicone membranes coated with isotropic (iso) fibronectin (FN) or micropatterned with FN (aniso), cultured statically for 1h (t=0h), and stretched for increasing amount of time (t=6h, 24h, 96h) before RNA extraction and hybridization on Affymetrix microarrays. For aniso tissues, stretch was applied in either the longitudinal (long) or transverse (trans) direction. RNA was collected over six primary NRVM harvests and thus RNA was also extracted and analyzed from samples seeded for 1h (at t=0h) on iso FN to be used to normalize across cell harvests.

2013-01-30 | E-GEOD-43846 | biostudies-arrayexpress

Expression data from static and cyclic stretched porcine aortic heart valves

Project description:We explored the hypothesis that Serotonin (5HT) receptor signaling, that can be enhanced with 5HT transporter blockade with Fluoxetine (Fluox), in the aortic valve may vary based upon the biomechanical activity of the aortic valve leaflet. We used Affymetrix microarrays to study gene expression profiling of Porcine Aortic Valves (PAV) incubated under organ culture conditions for 24 hours in either a static state or with 10% cyclic stretch, simulating physiologic leaflet motion. PAV in the bioreactor with or without stretch were exposed to 5HT along or the combination 5HT plus Fluox. Fresh porcine aortic valves were obtained from a local abattoir. The three leaflets were excised from each valve and a rectangular section of tissue 15x10 mm was isolated from the central region of each valve cusp. These samples were randomized and assigned to one of four groups. The experimental groups were: 1) Static conditions with no agents added; 2) Cyclic stretch conditions with no agents added; 3) Static conditions with 5HT plus Fluox added; and 4) Cyclic stretch conditions with 5HT plus Fluox added.

2010-05-27 | E-GEOD-21663 | biostudies-arrayexpress

Expression data of Gata4 endothelial cell-subtype specific knockout primary murine liver sinusoidal endothelial cells

Project description:Liver sinusoidal endothelial cells (LSEC) constitute discontinuous, permeable microvessels, with a characteristic program of gene expression that differs significantly from continuous microvascular endothelial cells e.g. in the lung. Gata4 is described as master regulator of LSEC specification during liver development. Here, we sought to analyze the role of endothelial Gata4 in the adult liver. We used microarrays to analyse the program of gene expression in murine liver endothelial cells with Gata4 deficiency.

2020-09-15 | GSE141001 | GEO

Differential expression of miRNA in stretched rat alveolar epithelial cells

Project description:Cyclic stretch of alveoli is characteristic of mechanical ventilation, and is postulated to be partly responsible for the lung injury and inflammation in ventilator induced lung injury. We propose that miRNAs may regulate some of the stretch response and, therefore, hypothesized that miRNAs would be differentially expressed between stretched and unstretched rat alveolar epithelial cells (RAECs).

2012-03-06 | GSE36256 | GEO

Genome-wide Expression Profiling and Pathway Analysis in Cyclic Stretched Human Trabecular Meshwork Cells

Project description:To identify differentially expressed miRNAs in human trabecular meshwork (HTM) cells in response to cyclic mechanical stretch

2020-02-01 | GSE113755 | GEO

Expression data from Notch intracellular domain overexpressing primary murine liver sinusoidal endothelial cells

Project description:Liver sinusoidal endothelial cells (LSEC) constitute discontinuous, permeable microvessels, with a characteristic programme of gene expression that differs significantly from continuous microvascular endothelial cells e.g. in the lung. Notch signalling plays a crucial role in a variety of processes in endothelial cells such as vascular morphogenesis, and vascular differentiation. We used microarrays to analyse the programme of gene expression in murine liver endothelial cells with enhanced Notch signalling.

2018-11-05 | GSE119875 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data