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Precise Gene Editing Preserves Hematopoietic Stem Cell Function Following Transient p53-Mediate DNA Damage Response [scRNA-seq]

ABSTRACT: We performed single-cell (sc)RNA-Seq on male donors FACS-sorted “primitive” (CD34+ CD133+ CD90+) and “progenitor” (CD34+ CD133+ CD90-) HSPC cells treated with IL2RG ZFN, High Specificity (HS), (Low Specificity (LS) or control RNP in a ratio 1 to 1. We also included HS RNP and the cognate repair template containing a GFP expression cassette delivered by AAV6 and sorted for targeted integration (HS/AAV6 GFP+ vs. HS/AAV6 GFP-)

ORGANISM(S): Homo sapiens

PROVIDER: GSE119594 | GEO | 2019/03/01

REPOSITORIES: GEO

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A distinctive DNA damage response in human hematopoietic stem cells reveals an apoptosis independent role for p53 in self-renewal

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