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Macrophage Ad5f35 M2 Challenge

ABSTRACT: Control untransduced or anti-HER2 CAR (Ad5f35 transduced) human macrophages were exposed to control media, human IL-4, or human IL-13, and the response to these cytokines was measured at a transcriptome-wide level. RNA was harvested 24 hours post cytokine exposure with Ambion RiboPure RNA Purification Kit (Thermo Fisher) and RNA-Seq libraries were generated using TruSeq RNA Library Prep Kit (Illumina). Libraries were sequenced on 75bp single-end reads using a NextSeq (Illumina).

ORGANISM(S): Homo sapiens

PROVIDER: GSE120086 | GEO | 2019/09/01

REPOSITORIES: GEO

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