Genomics

Dataset Information

16

Mammary Tissue: aCGH of primary (IGF-IR induced) and recurrent (IGF-IR independent) mammary tumors from MTB-IGFIR mice


ABSTRACT: Tumor recurrence represents a significant clinical challenge in the treatment and management of breast cancer. To investigate whether copy number aberrations (CNAs) facilitate the re-emergence of tumor growth from residual disease we performed array comparative genomic hybridization (aCGH) on primary and recurrent mammary tumors from an inducible mouse model of type-I insulin-like growth factor receptor (IGF-IR) driven breast cancer. This genome-wide analysis revealed primary and recurrent tumors harbored distinct copy number aberrations (CNAs) with relapsed tumors containing an increased number of gene-level gains and losses. Remarkably, CNAs detected in primary tumors were largely devoid of annotated cancer genes while the vast majority of recurrent tumors harbored at least one CNA containing a known oncogene or tumor suppressor. Specifically, a subset of recurrent tumors carried gains at 6qA2 and 9qA2 which encode the Met and Yap1 oncogenes respectively. The most frequent CNA detected was a focal deletion at 4qC5 involving the Cdkn2a and Cdkn2b tumor suppressor genes. Integrative analysis revealed positive correlations between gene copy number and mRNA expression suggesting Met, Yap1 and Cdkn2a/b may serve as potential driver genes that promote tumor recurrence. Together, these findings indicate that tumor recurrence is facilitated by the acquisition of CNAs with oncogenic potential and provide a framework to dissect the molecular mechanisms that mediate tumor escape from dormancy. Overall design: Two-color common reference design with 11 primary tumors (doxycycline-dependent) and 8 recurrent tumors (doxycycline-independent) from MTB-IGFIR mice.

INSTRUMENT(S): Agilent-014695 Mouse Genome CGH Microarray 244A (G4415A)(Probe Name version)

SUBMITTER: Roger Moorehead  

PROVIDER: GSE120186 | GEO | 2018-11-28

REPOSITORIES: GEO

Similar Datasets

2011-09-30 | E-GEOD-32152 | ArrayExpress
| GSE63306 | GEO
2006-01-25 | GSE4092 | GEO
2015-07-31 | E-MTAB-3110 | ArrayExpress
2015-07-31 | E-MTAB-3111 | ArrayExpress
2006-01-24 | E-GEOD-4092 | ArrayExpress
2012-10-01 | E-GEOD-37607 | ArrayExpress
| GSE63623 | GEO
2013-05-22 | E-GEOD-38323 | ArrayExpress
| GSE110221 | GEO