Screening of the targeted mRNAs of ER-anchored PARN by transcriptome sequencing.

ABSTRACT: The goals of this study are to identify the potential targets and then elucidate the function of ER-anchored PARN. After overexpressing the empty vector, PARN(wild type) or D28A(the enzyme-dead mutant of PARN) in HEK-293T cell respectively and the following fractionation of the cytosol and ER fraction, we performed the high-throughput RNA sequencing for each fraction. After comparing the abundance of detected transcripts in different samples we found ER-anchored PARN only affects a small subset of mRNAs in HEK-293T cell.

ORGANISM(S): Homo sapiens

PROVIDER: GSE120209 | GEO | 2021/09/19

REPOSITORIES: GEO