Genomics,Multiomics

Dataset Information

2

Identifying transcripts that are transcriptinoally regulated by CBFB and RUNX1 using RNAseq


ABSTRACT: Using RNAseq to identify differentially expressed transcripts between CBFB wild type (WT) and knockout (KO) or between RUNX1 wild type (WT) and knockout (KO) MCF10A cells. Overall design: Three repeats for CBFB KO and CBFB WT MCF10A cells. Four repeats for RUNX1 KO and RUNX1 WT MCF10A cells. One CBFB KO clone (#751) and two RUNX1 KO clones (5008 and 5010) were used.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Jing Huang  

PROVIDER: GSE120216 | GEO | 2019-04-18

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE120216_CBFB_KOvs_WT.csv.gz Csv
GSE120216_RUNX1_KO1vsWT.csv.gz Csv
GSE120216_RUNX1_KO2vsWT.csv.gz Csv
GSE120216_matrix_CBFB.xlsx Xlsx
GSE120216_matrix_RUNX1.xlsx Xlsx
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Publications


Translation and transcription are frequently dysregulated in cancer. These two processes are generally regulated by distinct sets of factors. The CBFB gene, which encodes a transcription factor, has recently emerged as a highly mutated driver in a variety of human cancers including breast cancer. Here we report a noncanonical role of CBFB in translation regulation. RNA immunoprecipitation followed by deep sequencing (RIP-seq) reveals that cytoplasmic CBFB binds to hundreds of transcripts and reg  ...[more]

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