Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The transcriptional fingerprint of satellite glial cells changes over time following peripheral nerve injury

ABSTRACT: Website for easy exploration of the data: https://rna-seq-browser.herokuapp.com/# For the first time, we here characterize the transcriptional signature of satellite glial cells (SGCs) in a nerve injury-paradigm by isolating SGCs from mouse dorsal root ganglia (DRGs) after peripheral nerve injury followed by next generation RNA sequencing (RNA-seq). We found that SGCs regulate their gene expression differently at 3 days and 14 days after peripheral nerve injury suggesting that they change their function over time. At both time points, we found downregulation of several genes linked to cholesterol. After 14 days we further detect regulation of genes linked to the immune system (MHC protein complex and leukocyte migration). The SGC RNA-seq data is accompanied by RNA-seq of purified nociceptors from injured (partial sciatic nerve ligation) or sham (ipsi after sham operation or contra after partial sciatic nerve ligation) L3-L5 DRGs.

ORGANISM(S): Mus musculus

PROVIDER: GSE120284 | GEO | 2020/01/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Comparative transcriptional analysis of the satellite glial cell injury response

Project description:The recent advance in single cell RNAseq technologies has enabled a new approach to investigate satellite glial cells (SGCs). Here we publish a dataset from mice subjected to sciatic nerve injury as well as a dataset from dorsal root ganglia cells after 3 days in culture. We use a meta-analysis approach to compare the injury response with that in other published datasets and conclude that SGCs share a common signature following sciatic nerve crush and sciatic ligation, involving transcriptional regulation of cholesterol biosynthesis. We also observed a considerable transcriptional change when culturing SGCs, suggesting that some differentiate into a specialised in vitro state, while others start resembling Schwann cell-like precursors.

2021-11-23 | GSE188971 | GEO

Entrapment Neuropathy Results in Different MicroRNAs Expression Patterns from Denervation Injury in Rats

Project description:To compare the microRNAs (miRNAs) expression profile in the innervated soleus muscle and L4-L6 DRG neuronsafter sciatic nerve entrapment with a non-constrictive silastic tube, subsequent surgical decompression, and denervation injury. The experimental soleus muscles and dorsal root ganglions (DRGs) from each experimental group (sham control, denervation, entrapment, and decompression) were analyzed with an Agilent® rat miRNA array to detect dysregulated miRNAs Three-condition experiment, DRGs and soleus muscles of the rats receiving sciatic nerve denervation 6 months, sciatic nerve entrapment 6 months, and sciatic nerve entrapment 6 months then decompression for 3 months v.s. soleus muscle (sham control), Biological replicates: 1 control replicates, 3 experiment replicates

2010-06-12 | E-GEOD-22181 | biostudies-arrayexpress

A Transcriptomic Analysis of Neuropathic Pain in Rat Dorsal Root Ganglia Following Peripheral Nerve Injury

Project description:In this study, we screened the differentially expressed genes (DEGs) in the dorsal root ganglia (DRG) from rats with sham or partial sciatic nerve ligation (pSNL) surgery 7 days using RNAseq technique to explore the molecular mechanisms of neuropathic pain

2020-06-30 | GSE117526 | GEO

Gene expression from aged and young sciatic nerve after nerve injury

Project description:Sciatic nerve ligation was performed on cohorts of 2-month and 24-month old animals. Resulting gene-expression data were generated from sciatic nerve 1 and 4 days after injury compared to naïve animals. Results show differences in sciatic nerve responses with normal aging. Total RNA taken from sciatic nerves from 2-month and 24-month old animals at either day 0, 1 and 4 after sciatic nerve crush injury.

2014-07-30 | E-GEOD-59040 | biostudies-arrayexpress

RNA-Seq of DRG and dorsal cord after nerve injury

Project description:Genes are up and down regualted in DRG and spinal dorsal cord after peripheral nerve injury WT male adult with sciatic and femoral nerve transection 7 days, RNA was purified from ipilateral or contralateral L4-L6 DRGs or lumbar spinal dorsal cords

2015-12-10 | E-GEOD-66619 | biostudies-arrayexpress

Glucocorticoid-regulated circadian expression genes in spinal cord of nerve-injured mice

Project description:We prepared adrenalectomized (ADX) and sham-operated (sham) mice kept under a light-controlled room (ZT, zeitgeber time; ZT0, light on; ZT12, light off), and thereafter their sciatic nerve was partially ligated; mice undergone partial sciatic nerve ligation (PSL) was used as an animal model of neuropathic pain. To screen pain-related genes whose spinal expression exhibits circadian oscillation in a glucocorticoid-dependent manner, we performed oligonucleotide microarray analyses using RNA isolated from the spinal cords of sham-PSL mice or ADX-PSL mice at ZT10 and ZT22 when circulating glucocorticoid levels in sham-PSL mice peaked and declined, respectively

2017-01-01 | GSE65756 | GEO

Gene expression from aged and young sciatic nerve after nerve injury

2014-07-30 | GSE59040 | GEO

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [dorsal root ganglia]

Project description:We applied Solexa sequencing technology to identify rat microRNA genes in dorsal root ganglia (DRGs) following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 114 novel miRNAs in rats were discovered and identified, of which 52 novel miRNAs were first reported in rat DRGs and 62 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration. 18-30 nt small RNAs from 30 Thirty Sprague-Dawley (SD) rats were sequenced at one Solexa lane

2012-05-06 | E-GEOD-25863 | biostudies-arrayexpress

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [dorsal root ganglia]

2012-05-06 | GSE25863 | GEO

Enhancer Analysis in Peripheral Nerve Injury

Project description:ChIP-seq of H3K27acetylation in sham and injured nerve. Schwann cells play an important role in the response of peripheral nerve to injury. This study was designed to identify enhancers that are altered in sciatic nerve at 3 days post-injury to help identify pathways that mediate the gene expression reprogramming that occurs in Schwann cells after nerve injury. We employed ChIP-seq analysis of H3K27 acetylation as a mark of actively engaged enhancers, and compared enhancers in the distal stump of transected sciatic nerve compared to contralateral (sham) condition.

2014-12-15 | GSE63103 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data