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Small RNA sequencing of LA-N-2 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

ABSTRACT: To examine the impact of gp130-mediated, pro-cholinergic neurokine intervention on the small RNA systems in human neuronal cells, we exposed cultured LA-N-2 cells to 100 ng/ml ciliary neurotrophic factor (CNTF). RNA sequencing of time points at 30/60 minutes and 2/4 days of CNTF exposure shows a significant response and an “immediate early”/”long term” dichotomy in differentially expressed miRNAs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE120520 | GEO | 2019/10/15

REPOSITORIES: GEO

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Small RNA sequencing of LA-N-2 and LAN-5 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

Project description:To examine the impact of gp130-mediated, pro-cholinergic neurokine intervention on the small RNA systems in female and male human neuronal cells, we exposed cultured LA-N-2 cells (female) to 100 ng/ml ciliary neurotrophic factor (CNTF), and LAN-5 cells (male) to 10 ng/ml CNTF. RNA sequencing of time points at 30/60 minutes and 2/4 days of CNTF exposure shows a significant response and an “immediate early”/”long term” dichotomy in differentially expressed miRNAs.

2019-10-15 | GSE132951 | GEO

Impacts of ciliary neurotrophic factor on the retinal transcriptome in a mouse model of photoreceptor degeneration

Project description:Ciliary neurotrophic factor (CNTF) has been tested in clinical trials for human retinal degeneration due to its potent neuroprotective effects in various animal models. To decipher CNTF-triggered molecular events in the degenerating retina, high-throughput RNA sequencing analyses were performed using the Rds/Prph2 (P216L) transgenic mouse as a preclinical model for retinitis pigmentosa. Retinal transcriptome data was obtained for untreated wild type retinas, Rds retinas treated with a lentivirus expressing CNTF from postnatal day 25 to 35, and Rds retinas similarly treated with a control lentivirus expressing GFP. Comparisons of transcriptome data detect significant changes between wild type and Rds retinas. In addition, RNA-seq data reveal elevation of transcripts in the innate immune system and growth factor signaling, as well as altered neuronal transmission and metabolism in CNTF-treated Rds retinas. These results demonstrate the influence of exogenous CNTF on the retinal transcriptome landscape and illuminate likely CNTF impacts in degenerating human retinas.

2022-10-25 | GSE216208 | GEO

Small RNA sequencing of LA-N-2 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

Project description:Small RNA sequencing of LA-N-2 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

| PRJNA493256 | ENA

Ciliary neurotrophic factor induces genes associated with inflammatory response and gliosis in the retina: A gene profiling study of flow-sorted, Muller (glial) cells

Project description:Our data suggest that CNTF remodels the transcription profile of Muller (glial) cells leading to induction of networks associated with transcription, cell cycle regulation and inflammatory response. CNTF also appears to function as an inducer of gliosis in the retina. These studies provide new insights into the biological functions of cytokines in the retina. Muller glial cells treated with CNTF for 1 and 3 days

2011-08-31 | E-GEOD-27195 | biostudies-arrayexpress

Small RNA sequencing of LA-N-2 and LAN-5 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

Project description:Small RNA sequencing of LA-N-2 and LAN-5 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

| PRJNA549516 | ENA

Ciliary neurotrophic factor induces genes associated with inflammatory response and gliosis in the retina: A gene profiling study of flow-sorted, Muller (glial) cells

Project description:Our data suggest that CNTF remodels the transcription profile of Müller (glial) cells leading to induction of networks associated with transcription, cell cycle regulation and inflammatory response. CNTF also appears to function as an inducer of gliosis in the retina. These studies provide new insights into the biological functions of cytokines in the retina.

2011-09-01 | GSE27195 | GEO

Neurotrophic factors and their receptors are altered by the mere partial IGF-1 deficiency

Project description:Neurotrophic factors (NTFs) are a relevant group of secreted proteins that modulate growth, differentiation, repair, and survival of neurons; playing a role in the maintenance of the synaptic unions, dendrites and axons; also, being crucial for peripheral nervous system development and regulating plasticity in the adult central nervous system. On the other hand, insulin-like growth factor 1 (IGF-1) has been ascertained multiple beneficial actions in the brain: neuro-development, -protection, -genesis and plasticity. To further investigate the possible mechanisms underlying IGF-1 deficiency in the establishment of neurological disease, microarray and RT-qPCR gene expression analyses coupled with in silico processing were performed in an experimental model of partial IGF-1 deficiency. Results show that the mere IGF-1 deficiency seems to be responsible for an altered expression of genes coding for neurotrophic factors (particularly ciliary neurotrophic factor and mesencephalic astrocyte-derived neurotrophic factor), their receptors and signaling pathways (specially RET). The presented findings support that IGF-1 deficiency might be involved in the establishment and progression of neurodegenerative disorders.

2018-12-29 | E-MTAB-7508 | biostudies-arrayexpress

GDE3 regulates oligodendrocyte precursor proliferation via release of soluble CNTFRα

Project description:Oligodendrocyte development is tightly controlled by extrinsic signals; however, mechanisms that modulate cellular responses to these factors remain unclear. Six-transmembrane glycerophosphodiester phosphodiesterases (GDEs) are emerging as central regulators of cellular differentiation via their ability to shed glycosylphosphatidylinositol (GPI)-anchored proteins from the cell surface. We show here that GDE3 controls the pace of oligodendrocyte generation by negatively regulating oligodendrocyte precursor cell (OPC) proliferation. GDE3 inhibits OPC proliferation by stimulating ciliary neurotrophic factor (CNTF)-mediated signaling through release of CNTFRα, the ligand-binding component of the CNTF-receptor multiprotein complex, which can function as a soluble factor to activate CNTF signaling. GDE3 releases soluble CNTFRα by GPI-anchor cleavage from the plasma membrane and from extracellular vesicles (EVs) after co-recruitment of CNTFRα in EVs. These studies uncover new physiological roles for GDE3 in gliogenesis and identify GDE3 as a key regulator of CNTF-dependent regulation of OPC proliferation through release of CNTFRα.

2019-09-06 | GSE135819 | GEO

ChIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

Project description:Whole genome profiling of histone methylation (H3K4me3, H3K27me3 and RNA Pol II) in Ciliary derived stem/progenitor cells and differentiated cells. In the present study we focus on the cellular differentiation that involves loss of pluripotency and gain of lineage and cell type-specific characteristics and we show developments from adult ciliary derived stem/progenitor cells to differentiated retinal neurons/glial cells of human cadaveric eyes in vitro. Ciliary pigment epithelial cells isolated from human cadaveric eye balls were cultured in the presence of mitogens like Epidermal Growth Factor (EGF) and Fibroblast Growth Factors (FGF) to generate neurospheres (Stem/progenitor population). The generated neurospheres were plated on laminin and poly-D-lysine-coated cover slips in the presence of the Brain derived neurotrophic factor (BDNF), Retinoic acid (RA) and 10% FBS for the differentiation (Retinal neuron/glial cells). Global Methylation patterns of histone H3K4me3, RNA Pol II and H3K27me3 were analysed in the present study. This work would provide an outline of epigenetic modifications in ciliary derived stem/progenitor cells and the progeny that underwent differentiation into retinal neurons/glial cells and present that specific histone methylations are involved in gene expression reprogramming during the process of differentiation.

2016-07-04 | E-GEOD-65268 | biostudies-arrayexpress

Transcription profiling of mouse rds mutant eye infected with CNTF/rAAV vs. control untreated eye

Project description:The retinal degeneration slow gene (rds/peripherin), is essential for normal photoreceptor (rods and cones) outer segment structure in the neurosensory retina. Our rds animal model, are stable transgenic mice that carry a null mutation (complete lost of function) in the rds gene. Those animals become blind, mimicking a human form of retinitis pigmentosa. In our experience we have observed dramatic levels of photoreceptor rescue after subretinal injection of those animals with a ciliary neurotrophic factor (CNTF) inserted into a recombinant Adeno-associate virus (rAAV) vector. However, this cell rescue is accompanied by changes in photoreceptor nuclear morphology, attenaution of the electroretinogram and evetually Apoptosis. By using Microarray analysis we hope to identify genes whose expression or lack thereof brings the cell to the brink of suicide. Identification of these genes will suggest therapeutic targets for the future. We will determine gene expression patterns in the retina of rds mice non-injected and injected with CNTF/rAAV virus after 60 days of injection. Our working hypothesis is that the injected CNTF/rAAV vector induces the expression of genes that somehow partially restores the photoreceptor morphology of rds mice. Paradoxically, it also has a negative effect on the phisiology of these cells (ERG). The up or down regulation of genes in different areas of the neurosensory retina collected by Laser Capture Microdissection will give us an insight on the relevance of candidate genes responsible for this phenomenon. We will use rds mice at age P25 and inject their rigth eye with CNTF/AVV virus (2.68x e13 particles/ml) and use the left eye as a control. Injection is performed via subretinal. Retinas will be collected at age P90 post injection. RNA will be extracted and processed accordingly for MIcroarray analysis.

2008-06-13 | E-GEOD-4756 | biostudies-arrayexpress

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