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SiRNAs 15-29 nucleotides long with 100 percent hit to Turnip mosaic virus clone GBR98

ABSTRACT: Mustard (Brassica juncea) was tested for Turnip mosaic virus infection. Small RNA of the plant was extracted and converted to DNA according to Ho, T., et al. (2006) Journal of Virological Methods 136:217-223, with primers modified to contain 454 adapter nucleotide sequences. The DNA then passed quality control through Bioanalyzer and Nanodrop before sequenced by 454 Life Sciences. Keywords: siRNA

ORGANISM(S): Turnip mosaic virus

PROVIDER: GSE12053 | GEO | 2008/09/12

SECONDARY ACCESSION(S): PRJNA113381

REPOSITORIES: GEO

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siRNAs 15-29 nucleotides long with 100 percent hit to Turnip mosaic virus clone GBR98

Project description:Mustard (Brassica juncea) was tested for Turnip mosaic virus infection. Small RNA of the plant was extracted and converted to DNA according to Ho, T., et al. (2006) Journal of Virological Methods 136:217-223, with primers modified to contain 454 adapter nucleotide sequences. The DNA then passed quality control through Bioanalyzer and Nanodrop before sequenced by 454 Life Sciences. Keywords: siRNA One sample analyzed by 454 high-throughput sequencing technology

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