Questioning the purity of the media - Extracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media

ABSTRACT: In the research field of extracellular vesicles (EVs), the use of EV-depleted fetal bovine serum (FBS) for in vitro studies is highly recommended to eliminate the confounding effects of media derived EVs. EV-depleted FBS may either be prepared by ultracentrifugation or bought commercially, nevertheless these depletion methods do not guarantee an RNA-free preparation. In this study we have addressed the RNA contamination issue in FBS, ultracentrifuged EV-depleted FBS, commercially available EV-depleted FBS, and also from our recently developed filtration based EV depleted FBS. Commercially available serum-free, xeno-free defined media were also screened for RNA contamination.

ORGANISM(S): other sequences Bos taurus

PROVIDER: GSE120594 | GEO | 2019/04/30

REPOSITORIES: GEO