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Ybx1 binding sites and Ybx1-binding m5C sites in zebrafish embryo sample.

ABSTRACT: The maternal-to-zygotic transition (MZT) is a conserved and fundamental process during which the embryo undergoes dramatic reprogramming to convert maternal environment to embryonic-driven programing. However, how the maternally supplied transcripts are dynamically regulated during MZT remains largely unknown. Herein, through genome-wide profiling of RNA 5-methylcytosine (m5C) in zebrafish early embryos, we show that m5C methylated maternal mRNAs display higher stability during MZT. We identify that the Y box-binding protein 1 (Ybx1) prefers to recognizing m5C-modified mRNAs through p-p interaction with a key residue Trp45 in its cold shock domain (CSD), which plays essential roles in maternal mRNA stability and early embryogenesis of zebrafish. Cooperated with an mRNA stabilizer Pabpc1a, Ybx1 promotes the stability of its target mRNAs in an m5C-dependent manner. Our study demonstrates a novel mechanism of RNA m5C methylation-regulated maternal mRNA stability during zebrafish MZT, highlighting the critical role of m5C mRNA methylation in early development.

ORGANISM(S): Danio rerio

PROVIDER: GSE120646 | GEO | 2019/08/07

REPOSITORIES: GEO

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Transcriptomics analysis of gene expression in wide type, ybx1 MO, pabpc1a MO zebrafish embryo sample.

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2017-03-27 | PXD006098 | Pride

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Ybx1 binding sites and Ybx1-binding m5C sites in zebrafish embryo sample.

Project description:Ybx1 binding sites and Ybx1-binding m5C sites in zebrafish embryo sample.

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