Genomics

Dataset Information

59

Acc1 determines memory potential of individual CD4+ T cells by regulating de novo fatty acid biosynthesis [RNA-seq]


ABSTRACT: Immunological memory is central to adaptive immunity and protection from disease. Changing metabolic demands as antigen-specific T cells transition from effector to memory have been well documented, but the cell-specific pathways and molecules that govern this transition are poorly defined. ACC1 is a rate-limiting enzym in fatty acid biosynthesis. We show that genetic deletion of ACC1 enhanced the formation of CD4+ T cell memory, and that inhibition of ACC1 function enhanced memory T cell formation during parasite infection in mice. ACC1-deficient effector Th cells had similar metabolic signatures to wild-type memory Th cells, and Acaca expression was inversely correlated with a memory gene signature in individual cells. Single cell analysis allowed us to identify a memory precursor-enriched population (CCR7hiCD137lo) present during early differentiation of effector CD4+ T cells. Thus, fatty acid metabolism directs cell fate determination during the generation of memory CD4+ T cells. Overall design: Phenotypic characterization of CCR7hiCD137lo and CCR7loCD137hi CD4+ T cell populations.

INSTRUMENT(S): Illumina HiSeq 1500 (Mus musculus)

SUBMITTER: Yusuke ENDO  

PROVIDER: GSE120667 | GEO | 2018-11-30

REPOSITORIES: GEO

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