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Single cell RNA-sequencing of innate lymphoid cells isolated from mouse skin layers

ABSTRACT: Purpose: Determine the layer-specific indentities and heterogeneity of innate lymphoid cells in the skin by single cell transcriptomic analysis Methods: Innate lymphoid cells were sorted from epidermis, dermis and subcutis of adult B6 mice and subjected to droplet based single-cell captureing and RNA isolation and library preparation. Results: 3,431, 3,356 and 1,061 innate lymphoid cells from epidermis, dermis, and subcutis were analyzed. Unsupervised clustering of the combined data set performed in Seurat identified 6 distinct ILC clusters. Conclusions: This study reveals skin layer-specific transcriptomic signature of innate lymphoid cells

ORGANISM(S): Mus musculus

PROVIDER: GSE120787 | GEO | 2018/10/04

REPOSITORIES: GEO

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RNA-sequencing of innate lymphoid cells that were sorted from mouse skin

Project description:Purpose: Transcriptomic analysis of skin innate lymphoid cells Methods: Innate lymphoid cells were sorted from epidermis, dermis and subcutis of adult B6 mice, respectively and subjected to RNA isolation and library preparation. Results: 3,078 differentially expressed genes (fold change>2 and p-value<0.05) were detected Conclusions: This study reveals transcriptome landscape of skin innate lymphoid cells

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