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Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [single hematopoietic cells (iHEC)]

ABSTRACT: We identified that the coordinated expression of exogenous Runx1 and Hoxa9 during the haematopoietic induction process from mouse PSC resulted in a type of induced hemogenic endothelial cells (iHEC). The single iHEC can be robustly educated into induced haematopoietic progenitor cells (iHPC), which gave rise to induced T cells (iT cells) with abundant TCRαβ repertoire in vivo. We sorted the single iHEC and iHPC from day-14, day-17, day-21 cell products derived from 241 iR9-ES and performed single-cell RNA-Seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE121371 | GEO | 2020/07/31

REPOSITORIES: GEO

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T cell regeneration from pluripotent stem cells by defined transcription factors Runx1 and Hoxa9 [single hematopoietic cells (iR9-iHEC&iR9-iHPC) II]

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| S-EPMC6951346 | biostudies-literature

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