Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of reprogramming of epigenome in TET1 knockdown cancer cell lines

ABSTRACT: Epigenetic changes associated with knockdown of TET1 in 2 cancer cell lines was assessed

ORGANISM(S): Homo sapiens

PROVIDER: GSE121648 | GEO | 2021/10/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

TET1 knockdown cancer cell lines

Project description:TET1 knockdown cancer cell lines

| PRJNA498002 | ENA

Analysis of reprogramming of epigenome in TET1 knockdown cancer cell lines

Project description:Analysis of reprogramming of epigenome in TET1 knockdown cancer cell lines

| PRJNA498005 | ENA

5hmC-seq in human MonoMac6 acute myeloid leukemia (AML) cells with and without knockdown of TET1

Project description:To dissect the mechanism underlying the regulation of TET1 on its target genes in AML, we performed 5hmC-sequencing for genomic DNA isolated from human MonoMac6 AML cells with and without knockdown of TET1.

2020-06-30 | GSE116549 | GEO

Analysis of tanscriptome reprogramming after knockdown of TET1 in cancer cell lines

Project description:The effect of modulating TET1 expression on cell phenotype in vitro was characterized

2021-10-22 | GSE121647 | GEO

Analysis of tanscriptome reprogramming after knockdown of TET1 in cancer cell lines

Project description:Analysis of tanscriptome reprogramming after knockdown of TET1 in cancer cell lines

| PRJNA498003 | ENA

The Role of Tet1 During Somatic Cell Reprogramming

Project description:Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed hMeDIP-seq and RNA-seq during somatic cells reprogramming with Tet1 over expression to invest the effect of Tet1.

2013-03-08 | GSE44935 | GEO

Analysis of DNA methylation in TET1 depleted colorectal cancer HCT116 cells

Project description:We aimed to analyze the relationship between TET1 and aberrant CpG methylation in colorectal cancer (CRC). We established two stable TET1 knockdown clones and negative control clones of HCT116 cells, and carried out DNA methylation analysis with HumanMethylation450 BeadChip.

2017-01-01 | GSE84399 | GEO

Analysis of DNA methylation in TET1 depleted colorectal cancer Colo320DM cells

Project description:We aimed to analyze the relationship between TET1 and aberrant CpG methylation in colorectal cancer (CRC). We established three stable TET1 knockdown clones and negative control clones of Colo320DM cells, and carried out DNA methylation analysis with HumanMethylation450 BeadChip.

2017-01-01 | GSE84397 | GEO

TET1 inhibits the migration and invasion of cervical cancer cells by regulating autophagy [MeDIP-Seq]

Project description:Methylation modifications play pertinent roles in regulating gene expression and various biological processes. The silencing of the demethylated modifier TET1 can affect the expressions of key oncogenes or tumor suppressor genes, thus contributing to tumor formation. Nonetheless, how TET1 affects the progression of cervical cancer is yet to be elucidated. In this study, we found that the expression of TET1 was significantly downregulated in cervical cancer tissues. Functionally, TET1 knockdown in cervical cancer cells can promote cell proliferation, migration, invasion, cervical xenograft tumor formation and EMT. On the contrary, its overexpression can reverse the aforementioned processes. Moreover, the autophagy level of cervical cancer cells can be enhanced after TET1 knockdown. Mechanistically, methylated DNA immunoprecipitation (MeDIP)-sequencing and MeDIP quantitative real-time PCR revealed that TET1 mediates the methylation of autophagy promoter regions. These findings suggest that TET1 affects the malignant biological behavior of cervical cancer cells by altering the methylation levels of autophagy genes NKRF and HIST1H2AK, but the specific mechanism needs to be investigated further.

2024-03-13 | GSE236315 | GEO

TET1 is a maintenance DNA demethylase that prevents methylation spreading in adult cells [DREAM]

Project description:We report that full length TET1 (TET1-FL) overexpression fails to induce global DNA demethylation in HEK293T cells. The preferential binding of TET1-FL to hypomethylated CpG islands (CGIs) through its CXXC domain leads to its inhibited 5-hydroxymethylcytosine (5hmC) production as methylation level increases. TET1-FL-induced 5hmC accumulates at CGI edges, while TET1 knockdown induces methylation spreading from methylated edges into hypomethylated CGIs. However, TET1 can regulate gene transcription independent of its dioxygenase catalytic function. Thus, our results identify TET1 as a maintenance DNA demethylase that does not purposely decrease methylation levels, but specifically maintains the DNA hypomethylation state of CGIs in adult cells.

2014-06-04 | GSE44038 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data