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Role of iron in the regulation of the epithelial-to-mesenchymal transition (RNA-seq)

ABSTRACT: The biological process termed Epithelial-to-Mesenchymal Transition (EMT) plays a central role in cancer cell invasion, metastasis, self-renewal and resistance to therapy1,2. Here, we characterize using RNA sequencing the mRNA levels changes occurring during EMT induced by epidermal growth factor in breast cancer MDA-MB-468 cells. 1. Nieto, M. A., Huang, R. Y., Jackson, R. A. & Thiery, J. P. EMT: 2016. Cell 166, 21–45 (2016). 2. Puisieux, A. & Brabletz, T. & Caramel, J. Oncogenic roles of EMT-inducing transcription factors. Nat. Cell Biol. 16, 488–494 (2014).

ORGANISM(S): Homo sapiens

PROVIDER: GSE121663 | GEO | 2019/10/01

REPOSITORIES: GEO

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CD44 regulates epigenetic plasticity by mediating iron endocytosis - part 2

Project description:The biological process termed Epithelial-to-Mesenchymal Transition (EMT) plays a central role in cancer cell invasion, metastasis, self-renewal and resistance to therapy(1,2). Here, using western blot technique, we show that H3K9me2 decreases when MDA-MB-468 breast cancer cells undergo EMT upon EGF. We validate this decrease by performing high-resolution MS/MS spectrum. Interestingly, we find that H3K9me2 is associated with mesenchymal genes regulation. 1. Nieto, M. A., Huang, R. Y., Jackson, R. A. & Thiery, J. P. EMT: 2016. Cell 166, 21–45 (2016). 2. Puisieux, A. & Brabletz, T. & Caramel, J. Oncogenic roles of EMT-inducing transcription factors. Nat. Cell Biol. 16, 488–494 (2014).

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A novel addressable 9600-microwell array single cell RNA-seq method applied on fresh mouse cortical cells and frozen human cortical nuclei

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CD44 regulates epigenetic plasticity by mediating iron endocytosis - part 1

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Role of iron in the regulation of the epithelial-to-mesenchymal transition (ChIP-seq)

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Transcription profiling by array of MDA-MB-231 cells with HMGA2 knock-down

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2012-02-13 | E-MEXP-3547 | biostudies-arrayexpress

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