Genomics

Dataset Information

0

ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis


ABSTRACT: The R-loop, composed of a DNA-RNA hybrid and the displaced single-stranded DNA, regulates diverse cellular processes. However, how cellular R-loops are sensed remain poorly understood. Here we report the discovery of the evolutionally conserved ALBA proteins (AtALBA1&2) functioning as the R-loops sensor of genic regions in higher plant Arabidopsis thaliana. AtALBA1 and 2 form a heterodimer protein complex and localize in the nucleus. While AtALBA1 binds to the DNA-RNA hybrid strand, AtALBA2 associates with single-stranded DNA in the R-loops. AtALBA1&2 preferentially binds to the genic R-loop regions that are associated with active epigenetic marks. Depletion of AtALBA1 or AtALBA2 results in hypersensitivity of plants to DNA damaging agents. Our results demonstrate the AtALBA1&2 protein complex is the Arabidopsis genic R-loops sensor for maintaining genome stability in Arabidopsis.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE121683 | GEO | 2019/04/17

REPOSITORIES: GEO

Similar Datasets

2019-04-17 | GSE124943 | GEO
2018-10-16 | GSE104067 | GEO
2021-11-25 | PXD024517 | Pride
2022-11-02 | PXD034331 | Pride
2021-02-22 | GSE156400 | GEO
2021-06-26 | GSE178905 | GEO
2021-09-17 | PXD022697 | Pride
2023-06-10 | GSE148581 | GEO
2022-05-22 | GSE147886 | GEO
2023-09-01 | GSE217878 | GEO