Transcriptomics,Genomics

Dataset Information

43

RIP-Chip analysis of Musashi (Msi1)


ABSTRACT: RIP-Chip analysis of Msi1 and identification of preferentially associated mRNAs. Keywords: RIP-Chip Overall design: Two experiments with different controls, GFP (Green Fluorescent Protein) and PABP1 (Poly A Binding Protein 1). Each experiment includes 4 microarrays, 2 replicates for each dye orientation.

INSTRUMENT(S): Agilent-012097 Human 1A Microarray (V2) G4110B (Feature Number version)

SUBMITTER: Luiz Penalva  

PROVIDER: GSE12171 | GEO | 2008-07-19

SECONDARY ACCESSION(S): PRJNA113461

REPOSITORIES: GEO

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Publications

Genomic analyses of musashi1 downstream targets show a strong association with cancer-related processes.

de Sousa Abreu Raquel R   Sanchez-Diaz Patricia C PC   Vogel Christine C   Burns Suzanne C SC   Ko Daijin D   Burton Tarea L TL   Vo Dat T DT   Chennasamudaram Soudhamini S   Le Shu-Yun SY   Shapiro Bruce A BA   Penalva Luiz O F LO  

The Journal of biological chemistry 20090303 18


Musashi1 (Msi1) is a highly conserved RNA-binding protein with pivotal functions in stem cell maintenance, nervous system development, and tumorigenesis. Despite its importance, only three direct mRNA targets have been characterized so far: m-numb, CDKN1A, and c-mos. Msi1 has been shown to affect their translation by binding to short elements located in the 3'-untranslated region. To better understand Msi1 functions, we initially performed an RIP-Chip analysis in HEK293T cells; this method consi  ...[more]

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