Genomics

Dataset Information

32

CRISPR screen for interferon-alpha inducible inhibitors of yellow fever virus replication


ABSTRACT: Host cells produce interferon (IFN) in response to viral infections. Secreted interferon results in the transcription and production of hundreds of interferon-stimulated genes (ISGs). A genome-wide CRISPR screen using IFN alpha-treated Huh7.5 cells was performed to determine which ISGs were required in order for host cells to suppress yellow fever virus (YFV) infection. Overall design: Human Huh7.5 cells were transduced with a human CRISPR library in a single replicate at 900X library coverage, treated with IFN alpha, infected with YFV-17D-venus, and GFP-positive cells that escaped IFN-alpha suppression of YFV-17D-venus infection were collected by FACS.

INSTRUMENT(S): Illumina NextSeq 500 (Homo sapiens)

SUBMITTER: John Schoggins  

PROVIDER: GSE121710 | GEO | 2018-10-25

REPOSITORIES: GEO

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Publications


The endoplasmic reticulum (ER) is an architecturally diverse organelle that serves as a membrane source for the replication of multiple viruses. Flaviviruses, including yellow fever virus, West Nile virus, dengue virus and Zika virus, induce unique single-membrane ER invaginations that house the viral replication machinery1. Whether this virus-induced ER remodelling is vulnerable to antiviral pathways is unknown. Here, we show that flavivirus replication at the ER is targeted by the interferon (  ...[more]

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