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Altered activity-dependent nascent transcription in Rai1-knockdown mature mouse cortical cultures

ABSTRACT: We performed the nascent RNA-seq technique, Bru-Seq, and chromatin immunoprecipitation sequencing (ChIP-seq) for Retinoic acid induced 1 (RAI1) using a custom antibody, on mouse neuronal cultures treated with control or Rai1-targeted shRNA, with 4 hour treatment of vehicle, tetrodoxin (TTX) or bicuculline (BIC) and 20 minute labeling of bromouridine.

ORGANISM(S): Mus musculus

PROVIDER: GSE121749 | GEO | 2020/06/20

REPOSITORIES: GEO

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