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Primary NKcells vs. NKAES-derived NK cells vs. NKcells stimulated by low/high dose IL2 after 7days of culture

ABSTRACT: Transcriptional profiling of NKAES-derived NK cells after 7 days of culture compared to primary human NK cells and NK cells stimulated by low or high dose IL2 after 7 days of culture.

ORGANISM(S): Homo sapiens

PROVIDER: GSE12198 | GEO | 2010/03/06

SECONDARY ACCESSION(S): PRJNA113421

REPOSITORIES: GEO

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Primary NKcells vs. NKAES-derived NK cells vs. NKcells stimulated by low/high dose IL2 after 7days of culture

Project description:Transcriptional profiling of NKAES-derived NK cells after 7 days of culture compared to primary human NK cells and NK cells stimulated by low or high dose IL2 after 7 days of culture. Four-condition experiment, primary NK cells vs. NKAES-derived NK cells after 7 days of culture vs. NK cells stimulated by low/high dose IL2 after 7 days of culture. Biological replicates: 5 control, 5 NKAES-derived NK cells, 3 NK cells stimulated by low dose IL2, 3 NK cells stimulated by high dose IL2 independently grown and harvested. One replicate per array.

2010-03-06 | E-GEOD-12198 | biostudies-arrayexpress

NK cell response to IL2 in vitro

Project description:Here we analyzed the transcriptional response to IL2 in NK cells in vitro. Splenic NK cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were directly cultured with IL2 (250U/ml) for 8hrs in vitro. Cells were then transferred directly into TriZol. RNA was prepared in Trizol for gene expression profiling by Affymetrix Mouse Gene 1.0 ST Arrays. The samples in this series are re-analyzed samples from GSE84562.

2016-07-20 | E-GEOD-84564 | biostudies-arrayexpress

NK cell response to IL2 in vitro

Project description:Here we analyzed the transcriptional response to IL2 in NK cells in vitro.

2016-07-20 | GSE84564 | GEO

Effect of JAK1/3 blockade on IL2 response in NK cells

Project description:IL2 signals are transmitted through JAK1 and JAK3, but the transcriptomic consequences of each to the overall response is unclear. Here we analyzed the relative contribution of JAK1 and JAK3 to the NK cell IL2 response in vitro using titrated doses of isoform specific JAK inhibitors. Blockade of JAK1 and JAK3 have unequal effects on IL2-induced transcripts at pharmacologically relevant doses. Splenic NK cells were isolated by negative selection from 6wk old male C57BL/6 mice. These were cultured with IL2 (250U/ml) in the presence or absence of JAK1/3 inhibitors for 8hrs in vitro. Cells were then transferred directly into TriZol. RNA was prepared in Trizol for gene expression profiling by Affymetrix Mouse Gene 1.0 ST Arrays.

2016-07-20 | E-GEOD-84562 | biostudies-arrayexpress

resting and IL2 activated human natural killer cells

Project description:The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Keywords: resting and IL2 activated NK cells(time series)

2007-08-07 | GSE8059 | GEO

Effect of JAK1/3 blockade on IL2 response in NK cells

2016-07-20 | GSE84562 | GEO

Transcription profiling of human resting and IL2 activated natural killer cells

Project description:The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Experiment Overall Design: RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturerâs instruction (GeneChipU133plus2Â® , Affymetrix Inc, CA).

2008-06-15 | E-GEOD-8059 | biostudies-arrayexpress

A novel membrane-bound IL-2 for NK cell immunotherapy

Project description:Transcriptional profiling of mbIL2 activated NK-92 cells compared to recombinant human IL2 activated NK-92 cells.

2022-10-21 | GSE199391 | GEO

Gene expression anaylsis of tumor stimulated human NK cells

Project description:Genome wide expression profiling of human NK cells stimulated with K562 erythroleukemic tumor cells after four hours of NK-tumor co-culture. Responding NK cells were compared to non-responding NK cells, delineated by display of CD107 on the NK cell surface following cytotoxic granule release. We hypothesized that tumor responses would initiate rapid changes in gene expression in the NK cell that would identify new features of the anti-tumor response of NK cells. Results identify NK cell activation responses and induction of TNF superfamily molecules with immunoregulatory activity. Human peripheral blood NK cells were co-cultured with tumor target cell line K562 for 4 hours with GolgiStop (brefeldin) then stained for granule exocytosis marker CD107a / CD107b, and NK cell markers then FACS sorted for responding NK cells (CD107+) and non-responding NK cells (CD107-). Pooled donor sample comprised NK cells from 3 individuals.

2014-03-18 | E-GEOD-55977 | biostudies-arrayexpress

Gene expression anaylsis of tumor stimulated human NK cells

2014-03-18 | GSE55977 | GEO

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