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RNA Sequencing Gene Expression Profile of IEL Incubated In The Presence or Absence of Osteopontin

ABSTRACT: We report the application of RNA-Seq technology for high-throughput gene expression profiling of intestinal intraepithelial lymphocytes (IEL) that were incubated for 24 hrs in the presence or absence of recombinant osteopontin.

ORGANISM(S): Mus musculus

PROVIDER: GSE122007 | GEO | 2018/11/01

REPOSITORIES: GEO

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RNA Sequencing Gene Expression Profile of IEL Incubated In The Presence or Absence of Osteopontin

Project description:RNA Sequencing Gene Expression Profile of IEL Incubated In The Presence or Absence of Osteopontin

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Project description:Intestinal intraepithelial lymphocytes (IEL) are an abundant population of tissue-resident T cells that protect the gut from pathogens and maintain intestinal homeostasis. The cytokine IL-15 is trans-presented by epithelial cells to IEL in complex with the IL-15 receptor α chain (IL-15Rα) and plays essential roles both in maintaining IEL homeostasis, and in inducing IEL activation in response to epithelial stress. When overproduced, IL-15 is a key driver of the gluten-induced enteropathy Coeliac disease, through cytotoxic activation of IEL. To better understand how IL-15 directly regulates both homeostatic and inflammatory functions of IEL, we performed quantitative proteomics of IL-15/Rα-stimulated murine IEL, sorted into their 3 main subpopulations, TCRγδ CD8αα, TCRαβ CD8αβ and TCRαβ CD8αα expressing IEL. The data reveal that high IL-15/Rα stimulation licenses cell cycle activation, upregulates the biosynthetic machinery in IEL, increases mitochondrial respiratory capacity and induces expression of cell surface immune receptors and adhesion proteins that potentially drive IEL activation.

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Osteopontin vs empty vector

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Gene expression analysis of CD122HI versus CD122LO Vγ7+ murine small intestinal IEL at 14-17 days of age

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In vitro activation of Btnl-1 transduced MODE-K cells with different stimuli

Project description:The experiment was designed to assess how intestinal epithelial cells respond to different soluble factors produced by intraepithelial lymphocytes (IEL) and how this response is altered when the epithelial cells express Btnl1. Total RNA of MODE-K cells transduced with Btnl1-GFP or GFP alone and stimulated with TNF+IL-1_ in comparison to medium and with supernatant from anti-CD3-activated IEL in comparison to supernatant from unstimulated IEL

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