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Cell wall mediated multi-cellular development during cotton fiber elongation

ABSTRACT: Transcriptional profiling of cotton fibers during development. Time course at 6 days post anthesis (dpa), 10 dpa, 20 dpa, and 24 dpa. All referenced to 20 dpa.

ORGANISM(S): Gossypium hirsutum

PROVIDER: GSE12202 | GEO | 2009/04/16

SECONDARY ACCESSION(S): PRJNA113429

REPOSITORIES: GEO

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Cell wall mediated multi-cellular development during cotton fiber elongation

Project description:Transcriptional profiling of cotton fibers during development. Time course at 6 days post anthesis (dpa), 10 dpa, 20 dpa, and 24 dpa. All referenced to 20 dpa. Four time points, three biological replicates with one technical replicate (dye swap) each. All referenced to 20 dpa. 18 arrays total.

2009-04-16 | E-GEOD-12202 | biostudies-arrayexpress

Identification and comparison of miRNAs involved in cotton fiber elongation between sea-island and upland cotton

Project description:To identify potential miRNAs involved in fiber development and elucidate their expression differences between G. barbadense and G. hirsutum, we constructed two small RNA libraries, Gb10 and Gh10, prepared from fibers of 3-79 (G. barbadense) and TM-1 (G. hirsutum) collected at 10 days post-anthesis (DPA). We identified 28 conserved miRNA families, including 24 that exactly match known plant miRNA families in miRBase. With MIREAP and newly developed software miRsearcher, 7 candidate-novel miRNAs were found. 5 candidate-novel miRNAs were expressed in both species, 2 candidate-novel miRNAs were expressed only in one species. Moreover, 4 miRNA families showed significant expression differences between sea-island cotton and upland cotton in 10 DPA fibers. two examples including 3-79 and TM-1 10 DPA fibers

2010-12-03 | E-GEOD-25773 | biostudies-arrayexpress

Cotton fiber LC-MS/MS - Proteomic Analysis of Differences in Fiber Development between Wild and Cultivated Gossypium hirsutum L

Project description:Upland cotton (Gossypium hirsutum L.) is one of the world’s most important fiber crops, accounting for more than 90% of all cotton production. While their wild progenitors have relatively short and coarse, often tan-colored fibers, modern cotton cultivars possess longer, finer, stronger, and whiter fiber. In this study, the wild and cultivated cottons (YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis (DPA), 20 DPA and mature stages at the physiological level. In order to explore the effects of domestication, reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality, an iTRAQ-facilitated proteomic methods were performed on developing fibers. There were 6990 proteins identified, among them 336 were defined as differentially expressed proteins (DEPs) between fibers of wild versus domesticated cotton. The down- or up-regulated proteins in wild cotton were involved in Phenylpropanoid biosynthesis, Zeatin biosynthesis, Fatty acid elongation and other processes. Association analysis between transcroptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 DPA. The difference of proteomics had been verified at the mRNA level by qPCR, also at physiological and biochemical level by POD activity determination and ZA content estimation. This work corroborate the major pathways involved in cotton fiber development and demonstrate that POD activity and zeatin content have a great potential related to fiber elongation and thickening.

2017-08-28 | PXD006604 | Pride

Identification and comparison of miRNAs involved in cotton fiber elongation between sea-island and upland cotton

2010-12-03 | GSE25773 | GEO

Control of Fiber Color in Naturally Colored Cotton by Flavonoid Biosynthesis

Project description:Compare the expression profiles of natural brown and green cotton fibers, and white color fiber at 0 and 12 days post-anthesis (DPA) to identify genes involved in pigment synthesis

2017-11-28 | GSE76400 | GEO

Comparison of cotton fiber to ovule at 9 DPA

Project description:RNAs from the upland cotton 9-DPA fibers were compared to the 9-DPA fiber-detached ovule. RNAs from the upland cotton 9-DPA fibers were compared to the 9-DPA fiber-detached ovule.

2010-07-01 | E-GEOD-4642 | biostudies-arrayexpress

Comparison of cotton fiber to ovule at 9 DPA

Project description:RNAs from the upland cotton 9-DPA fibers were compared to the 9-DPA fiber-detached ovule. Keywords: tissue comparison

2006-04-12 | GSE4642 | GEO

Cotton Fiber Development Time-course Analysis

Project description:To get glance to the events of the fast elongation and second cell wall synthesis stages of developing cotton fiber cells, we examined expression patterns of over 5000 genes by cDNA array from 3 to 18 DPA in a 3-day interval. RNAs from the 3-, 6-, 9-, 12-, 15-, and 18-DPA fibers were compared to the 9-DPA fiber RNA for time-course analysis. Four biological repeats were carried out including two dye-swap ones.

2006-04-12 | E-GEOD-4639 | biostudies-arrayexpress

non-coding RNA sequencing of upland cotton fiber and ovule

Project description:Three samples of cotton fiber harvested 3, 7 and 15 days post anthesis (DPA) and a sample of cotton ovule harvestd 0 DPA were used for non-coding RNA extraction and seloxa sequencing experiments, respectively.Cotton plants (Gossypium hirsutum cv. Xuzhou 142) were grown in a soil mixture in fully automated walk-in growth rooms with 300 M-BM-5mol mM-bM-^@M-^S2 sM-bM-^@M-^S1 average light intensity, 60% relative humidity and temperatures set to 30C during the light period and 28C during the dark (12-h light/dark cycle). These conditions were consistent throughout the year. Small RNA molecules under 30 bases were amplified and isolated from an agarose gel. The purified DNA was used directly for cluster generation and sequencing analysis using the Illumina Genome Analyzer IIx according to the manufacturer's instructions. The 38nt sequence tags from sequencing went through data cleaning first, which included getting rid of the low-quality tags and clipping adapter sequences. Three samples of cotton fiber harvested 3, 7 and 15 days post anthesis (DPA) and a sample of cotton ovule harvestd 0 DPA were used for non-coding RNA extraction and seloxa sequencing experiments, respectively.

2012-06-06 | E-GEOD-37125 | biostudies-arrayexpress

Transcriptomics analyses of Gossypium hirsutum fiber in wild-type and immature fiber mutant

Project description:We performed a comparative genomics approach between im mutant and TM-1 in order to understand the function of im gene reducing the degree of fiber cell wall development. We compared transcriptome profiles of developing fibers (10, 17, and 28 days post anthesis (DPA)) between two NILs using Affymetrix cotton array chip containing 21,854 transcripts.

2014-05-01 | E-MEXP-3901 | biostudies-arrayexpress

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