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Integrated transcriptional regulatory network of quorum sensing, replication, and SOS response in Dinoroseobacter shibae

ABSTRACT: Transcriptional response of the QS-negative D. shibae strain ∆luxI1 towards externally added 500nM autoinducer (AI) 3-oxo-C14-HSL over a time period of 3 hours.

ORGANISM(S): Dinoroseobacter shibae

PROVIDER: GSE122111 | GEO | 2018/11/03

REPOSITORIES: GEO

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Response to different N-acyl homoserine lactones and N-acyl homoserine lactones-pretreated plants to secondary challenge with flg22.

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ALI1 interacts with N-acyl homoserine lactone and is involved in the perception of the quorum sensing molecule

Project description:In this report, we investigated oxo-C14-HSL-interacting proteins in plants. We propose the AHL-Interacting Protein 1 (ALI1) (previously known as AtGalk2) as interacting protein for oxo-C14-HSL in Arabidopsis. We present here several levels of confirmation and suggest that ALI1 is required for the perception of oxo-C14-HSL in Arabidopsis. This is the first direct evidence of a plant protein interacting with a bacterial quorum sensing molecule.

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Response to different N-acyl homoserine lactones and N-acyl homoserine lactones-pretreated plants to secondary challenge with flg22.

Project description:In order to gain coherent insights into plant responses we performed transcriptional analysis of Arabidopsis seedling after treatment with three different AHLs: N-hexanoyl-L-homoserine lactone (C6-HSL), N-3-oxo-decanoyl-L-homoserine lactone (oxo-C10-HSL), and N-3-oxo-tetradecanoyl-L-homoserine lactone (oxo-C14-HSL). Furthermore, we analyzed the transcriptome of oxo-C14-HSL pretreated plants after a secondary challenge with 100 nM flg22 for 2 and 24 hours after treatment. Gene expression in Arabidopsis thaliana seedlings were measured after a 3-days-pretreatment with 6 ÂµM of three different N-acyl homoserine lactones in comparison to plants treated with the coresponding volume of acetone (solvent control). Plants pretreated with oxo-C14-HSL were in addition treated with 100nM of flg22 to induced defense response. Three independent experiments were performed (replicates).

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Project description:Dinoroseobacter shibae overview

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Project description:D. shibae was cultivated under changing light regimes and samples for transcriptome and metabolome were taken.

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