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Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila

ABSTRACT: We investigated tissue-specific regulation of gene expression in a long lived Drosophila IIS mutant (dilp2-3,5) compared to its control (wDah). The longevity of dilp2-3,5 mutants only occurs in the presence of the endosymbiotic bacteria Wolbachia. We therefore use flies (10 day old) in the presence (wDah and dilp2-3,5) or absence (wDahT and dilp2-3,5T) of Wolbachia to identify the changes in gene expression that could potentially be causal for longevity. We quantified gene expression for all experimental groups, concurrently on the level of the proteome and the transcriptome and in four key tissues: brain, gut, fat body, and muscle. Transcriptome quantification was carried out on three biological replicates per experimental group. Corresponding proteome quantification was carried out on five biological replicates per experimental group, and published on PRIDE (accession number PXD011589).

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE122190 | GEO | 2021/04/21

REPOSITORIES: GEO

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Project description:Globally invasive Aedes aegypti mosquitoes disseminate numerous arboviruses that impact human health. One promising method to control Ae. aegypti populations is transinfection with the intracellular bacterium Wolbachia pipientis, a symbiont that naturally infects ~40-52% of insects but is normally absent from Ae. aegypti. Transinfection of Ae. aegypti with the wMel Wolbachia strain induces cytoplasmic incompatibility (CI), allowing infected individuals to rapidly invade native populations. Further, wMel Wolbachia-infected females display refractoriness to medically relevant arboviruses. Thus, wMel Wolbachia-infected Ae. aegypti are being released in several areas to replace native populations, thereby suppressing disease transmission by this species. Wolbachia is reported to have minimal effects on Ae. aegypti fertility, but its influence on other reproductive processes is unknown. Female insects undergo several post-mating physiological and behavioral changes required for optimal fertility. Post-mating responses (PMRs) in female insects are typically elicited by receipt of male seminal fluid proteins (SFPs) transferred with sperm during mating, but can be modified by other factors, such as adult age, nutritional status, and microbiome composition. To assess how Wolbachia infection influences Ae. aegypti female PMRs, we collected wMel Wolbachia-infected Ae. aegypti from the field in Medellín, Colombia and introduced the bacterium into our laboratory strain. We found that Wolbachia influences female fecundity, fertility, and re-mating incidence. Further, we observed that Wolbachia significantly extends longevity of virgin females. Changes in female PMRs are not due to defects in sperm transfer by infected males, or sperm storage by infected females. Using proteomic methods to examine the seminal proteome of infected males, we found that Wolbachia infection has a moderate effect on SFP composition. However, we identified 125 Wolbachia proteins that are paternally transferred to females by infected males. Surprisingly, the CI factor proteins (Cifs), were not detected in the ejaculates of Wolbachia-infected males. Our findings indicate that Wolbachia infection of Ae. aegypti alters female post-mating responses, potentially influencing control programs that utilize Wolbachia-infected individuals.

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Profiling tissue-specific modulation of gene expression in response to insulin signalling in Drosophila

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