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Neonatal and embyronic CNS of mice with maternal or paternal duplication of proximal chromosomes 7 and 15 (430B)

ABSTRACT: Gene expression profiling was performed on CNS tissue from neonatal mice carrying the T9H translocation and maternal or paternal duplication of proximal Chromosomes 7 and 15. Our analysis revealed the presence of two novel paternally expressed intergenic transcripts at the PWS/AS locus. The transcripts were termed Pec2 and Pec3 for paternally expressed in the CNS.Our analysis also revealed imprinting of Magel2, Mkrn3, Ndn,Ube3a and Usp29, as well as Pec2 and Pec3 in embryonic brain, 15.5 dpc, and provided a survery of biallelically expressed genes on proximal Chromosomes 7 and 15 in embryonic and neonatal CNS. Keywords: genetic modification, developmental comparison

ORGANISM(S): Mus musculus

PROVIDER: GSE12230 | GEO | 2008/07/25

SECONDARY ACCESSION(S): PRJNA113863

REPOSITORIES: GEO

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Project description:We performed gene expression microarray analysis of the hypothalamic response to starvation in neonatal wild-type mice, and in Snord116del mice that are a mouse model for PWS. This study is motivated by the neonatal feeding problems observed in several genetic diseases including Prader-Willi syndrome (PWS). Later in life, individuals with PWS develop hyperphagia and obesity due to lack of appetite control. We hypothesize that failure to thrive in infancy and later-onset hyperphagia may be related and could be due to a defect in the hypothalamus. In this study, we performed gene expression microarray analysis of the hypothalamic response to starvation in neonatal wild-type mice, and in Snord116del mice that are a mouse model for PWS. The neonatal starvation response was dramatically different from that reported in adult rodents. Genes that are affected by adult starvation are not changed in the hypothalamus of 5 day-old pups that were starved for 6 hrs. Unlike in adult rodents, expression levels of Nanos2 and Pdk4 were increased, and those of Pgpep1, Ndph, Brms1l, Mett10d, and Snx1 were decreased after fasting. In addition, we compared hypothalamic gene expression profiles at days 5 and 13 to document developmental changes. Notably, the gene expression profiles of Snord116del deletion mice and wild-type littermates were very similar at both postnatal days 5 and 13, and after starvation. We compared hypothalamic RNA between Snord116del mice with wild type (C57BL/6J) littermates at P5 and P13, with or without fasting treatment. We are particularly interested in the following comparisons: Comparison 1: wild type vs. PWS deletion in P5 non-starved mice; Comparison 2: wild type vs. PWS deletion in P5 fasting mice; Comparison 3: non-starved vs. fasting in P5 wild type mice; Comparison 4: non-starved vs. fasting in P5 PWS deletion mice; Comparison 5: wild type vs. PWD deletion in P13 non-starved mice; Comparison 6: P5 wild type vs. P13 wild type mice;

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Neonatal and embyronic CNS of mice with maternal or paternal duplication of proximal chromosomes 7 and 15 (430B)

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