Genomics

Dataset Information

0

Dissociating fibrosis from inflammation in systemic sclerosis – the case of interleukin-17A


ABSTRACT: Objectives. Interleukin-17A (IL-17A) levels are increased in SSc skin and other organs but its role in fibrosis development is highly debated. Since epithelial cells are preferential targets of IL-17A, we aimed at investigating the role of IL-17A in the interactions between epidermis and dermis. Methods. Organotypic cultures of HD full human skin were challenged with IL-17A,TNF and TGF-β. Inflammatory mediators and type I collagen (col-I) levels were quantified. IL-17A- and TGF-β-induced changes in gene expression in full human skin were analysed by RNA sequencing. Results. In full human skin, TGF-β induced pro-fibrotic gene signature dominated by Wnt signalling. While IL-17A strongly promoted expression of many pro-inflammatory genes, it did not affect collagen gene levels but decreased Wnt signalling. At the protein level, IL-17A showed direct anti-fibrotic effects, as well as decreased by 2-fold TGF-β-triggered col-I production. Conclusions. We report here firstly, a novel model of fibrotic skin and secondly, that IL-17A acts as a potent anti-fibrotic factor in the full human skin. Furthermore, we show that IL-17A not only decreased ECM deposition by itself, but also counteracted TGF-β pro-fibrotic activities. Thus, IL-17A seems to play a dual role in SSc skin – strongly pro-inflammatory but anti-fibrotic, being an example that fibrosis and inflammation, although closely related, are two different processes. These data may help in directing and interpreting therapeutic approaches in SSc, since both, IL-17A and TGF-β, are target candidates in clinical trials.

ORGANISM(S): Homo sapiens

PROVIDER: GSE122305 | GEO | 2020/02/11

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-69447 | biostudies-arrayexpress
2016-04-14 | GSE69447 | GEO
| E-GEOD-53795 | biostudies-arrayexpress
2023-12-06 | GSE249279 | GEO
| E-GEOD-53751 | biostudies-arrayexpress
| E-GEOD-33581 | biostudies-arrayexpress
2020-01-14 | MSV000084800 | MassIVE
2023-01-29 | GSE206473 | GEO
2014-09-25 | GSE53795 | GEO
2018-10-02 | GSE115503 | GEO