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Single-cell RNA-seq reveals the molecular progression of retinal progenitors in human embryonic stem cell models of early retinogenesis

ABSTRACT: We used single-cell RNA-sequencing (scRNA-seq) on human embryonic stem cell (hESC)-derived retinal organoid to deconstruct RPC developmental trajectory in the early retinogenesis, which revealed the fate branchpoint in RPC progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE122783 | GEO | 2019/08/22

REPOSITORIES: GEO

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Bankable human iPSC-derived Retinal Progenitors as a New Source of Multipotent Cells

Project description:Retinal progenitor cells (RPCs) are the source of all retinal cell types during retinogenesis. Until now, the isolation and expansion of RPCs has been at the expense of their multipotency. Here, we report simple methods and media for the generation, expansion, and cryopreservation of human induced pluripotent stem-cell derived-RPCs (hiRPCs). Thawed and passed hiRPCs maintained biochemical and transcriptional RPC phenotypes and their ability to differentiate into all retinal cell types. Specific conditions allowed the generation of large cultures of photoreceptor precursors enriched up to 90% within a few weeks and without a purification step. Combined RNA-seq analysis between hiRPCs and retinal organoids identified genes involved in developmental or degenerative retinal diseases. Thus, hiRPC lines could provide a new source of retinal cells for cell-based therapies or drug discovery and could be an advanced cellular tool to better understand retinal dystrophies.

2023-08-08 | GSE220792 | GEO

Mettl3 regulates late retinogenesis

Project description:Mettl3 was conditionally knocked out from retinal progenitor cells. Metll3 deficient retinas exhibited disrupted cell cycle during late retinogenesis and abnormality in retinal architecture and physiology.

2022-12-02 | GSE181095 | GEO

Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [TEBS_Mm]

Project description:Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [TEBS_Mm]

| PRJNA431784 | ENA

Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [ChIP-seq_Mm]

Project description:Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [ChIP-seq_Mm]

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Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [RNA-Seq_Mm]

Project description:Retinal Cell Type Epigenetic Memory Predicts Reprogramming Efficiency and Retinogenesis in 3D Organoid Cultures [RNA-Seq_Mm]

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Mettl3 regulates late-stage retinogenesis

Project description:To study the function of Mettl3 during retinal development, we used Six3-Cre and Mettl3floxed mice to conditionally knock out Mettl3 from retinal progenitor cells. The mutant mice show defects in late-stage retinogenesis and structural and physiological homeostasis of the retina.

2022-12-03 | GSE180813 | GEO

Transcriptomic analysis of progenitor marker expression in retinal cells

Project description:We reported that the cells expressed early RPC markers and late RPC markers at high level.

2018-12-26 | GSE116955 | GEO

DNA methylation in hESC derived brain organoids and iPSC derived neurons, astrocytes and neural progenitors

Project description:We studied the impact of ZIKA virus infection on DNA methylation in whole organoids, organoid derived astrocytes, neurons neural progeniotors and hESC or iPSC derived astrocytes, neurons, neural progenotor cells

2018-01-12 | GSE109104 | GEO

Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.

Project description: Not available

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Origin of kidney papillary tumors from renal progenitors

Project description:Purpose: Using single-cell RNA sequencing (scRNAseq), Young et al. (Science, 2018, PMID 30093597) recently identified a canonical cancer transcriptome as the possible cell of origin of papillary renal cell carcinoma (pRCC) that matched a rare proximal convoluted tubular cell population characterized as VCAM1+ SLC17A3+ SLC7A13-, a cell cluster signature defined as PT1. The goal of this study is to compare pRCC transcriptome to human renal progenitor cell (RPC) transcriptome. Methods: To compare the pRCC cells with PT1 features to our RPC population, we analyzed Young pRCC scRNAseq data set together with scRNAseq data obtained from human RPC cultures. Results: The PT1 signature that characterizes the cell of origin of pRCC identifies a RPC cluster. Conclusions: These results suggest that the cell of origin of pRCC corresponds to RPCs.

2020-07-16 | GSE137620 | GEO

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