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The genes differentially expressed in human colon cancer cells LoVo stably transfected with LV-control and LV-miR-500a respectively.

ABSTRACT: Microarrays were used to screen differentially expressed genes in LoVo stably transfected with LV-control and LV-miR-500a.

ORGANISM(S): Homo sapiens

PROVIDER: GSE122884 | GEO | 2019/02/27

REPOSITORIES: GEO

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The genes differentially expressed in human colon cancer cells LoVo stably transfected with LV-control and LV-miR-500a respectively.

Project description:The genes differentially expressed in human colon cancer cells LoVo stably transfected with LV-control and LV-miR-500a respectively.

| PRJNA506790 | ENA

Transcriptomics revealed the putative mechanism underlying the impact of HFL1 fibroblasts with overexpression of GALC on LoVo cell

Project description:Purpose:To elucidate the mechanism by which overexpression of GALC senescent fibroblasts promote the tumor properties of LoVo CRC cells Methods: LoVo cell which co-cultured with LV-GALC HFL1 fibroblasts cells and LoVo cell which co-cultured with LV-NC HFL1 fibroblasts cells using Transwell chamber were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: Our study represents the first detailed analysis of the impact of LV-GALC HFL1 fibroblasts and LV-NC HFL1 fibroblasts on LoVo cell with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles.We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.Fibroblasts regulate the tumorigenicity of CRC cells and play important roles in tumor biology.

2020-02-25 | GSE145662 | GEO

Expression data from LoVo colon cancer lines +/- constitutive LIN28B expression

2010-12-29 | GSE26334 | GEO

Expression data from LoVo colon cancer lines +/- constitutive LIN28B expression

Project description:We sought to elucidate the molecular mechanisms whereby LIN28B functions by comparing the gene expression profile of cells constitutively expressing LIN28B to empty vector controls. Accordingly, we performed microarray analysis on total RNA isolated from empty vector LoVo and LIN28B-expressing LoVo colon cancer cell lines. Constitutive LIN28B expression was achieved in the LoVo (ATCC #CCL-229) colon cancer cell line via retroviral transduction of MSCV-PIG-LIN28B. Contol = empty vector MSCV-PIG.

2010-12-29 | E-GEOD-26334 | biostudies-arrayexpress

microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

Project description:LoVo cells were cultured in sEV-depleted (160,000xg, 16h) complete medium and the supernatant were collected after 72h. sEVs were purified and centrifuged at 100,000xg for 2.5h using a Beckman SW41Ti rotor to know the miRNA in LoVo cells and LoVo-Small Extracellular Vesicles

2019-07-01 | GSE117866 | GEO

Expression data from xenograft tumors derived from LoVo colon cancer lines +/- constitutive LIN28B expression

2010-12-29 | GSE26335 | GEO

Gene expression changes with LGR5 knockdown in LoVo colon cancer cells

Project description:The goal of this study was to identify gene expression changes in response to loss of LGR5 in the LoVo colon cancer cell line.

2019-08-13 | GSE135749 | GEO

Gene expression profiling in LoVo-P compared with LoVo-C cells

Project description:The differential expression of mRNA in LoVo-P cells compared with LoVo-C cells was successfully detected using the Arraystar Human LncRNA/mRNAArray V4.0, that used for the global profiling of 20730 human mRNA and 40173 long non-coding RNA (LncRNA) transcripts.

2017-09-20 | GSE103989 | GEO

Expression profiling of SMMC-7721 cells: stably expressing lncRNA-hPVT1 vs LV-Control

Project description:In our present study, we found that lncRNA-hPVT1 could promote HCC cell proliferation. We want to know what were the target genes of lncRNA-hPVT1. So we constructed the hPVT1-overexpressed SMMC-771 cells and observed the mRNA profile in hPVT1-overexpressed and control SMMC-771 cells. lncRNA-hPVT1 induced transcriptional changes in SMMC-7721. Two-condition experiment, control (LV-Control) vs lncRNA-hPVT1 expression (LV-hPVT1 Clone2 ). Each has 3 biological repeats.

2014-05-20 | E-GEOD-57786 | biostudies-arrayexpress

Expression data from xenograft tumors derived from LoVo colon cancer lines +/- constitutive LIN28B expression

Project description:We sought to elucidate functions of LIN28B and potential mechanisms whereby it may promote metastasis by comparing the gene expression profile of LIN28B metastases to primary tumors. Accordingly, we performed microarray analysis on total RNA isolated from empty vector tumors, LIN28B-LoVo tumors, and LIN28B-LoVo metastases Constitutive LIN28B expression was achieved in the LoVo (ATCC #CCL-229) colon cancer cell line via retroviral transduction of MSCV-PIG-LIN28B. xenografts were produced via injection of 1x106 cells subcutaneously into the rear flanks of nude mice. Microarrays were conducted on primary tumors from empty vector and LIN28B-expressing cells, as well as metastases derived from primary tumors constitutively expressing LIN28B. (Note: metastases did not occur with empty vector tumors)

2010-12-29 | E-GEOD-26335 | biostudies-arrayexpress

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