Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE123030 | GEO | 2018/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing.

Project description: Not available

| S-EPMC6336504 | biostudies-literature

Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing

Project description:Developmental heterogeneity of microglia and brain myeloid cells revealed by deep single-cell RNA sequencing

| PRJNA507281 | ENA

Deep single-cell RNAseq of microglia and brain myeloid cells from various brain regions and developmental stages

Project description:We generated single-cell RNAseq profiles of microglia and brain myeloid cells (1922 total; 1816 cells passed quality control) from different developmental stages (E14.5, P7 and P60) to study their heterogeneity. Cells were isolated from either the whole developing brain (for the E14.5 stage) or six separate regions (for P7 and P60 stages): Cortex (CTX), Cerebellum (CB), Hippocampus (HIP), Striatum (STR), Olfactory bulb (OB), Choroid plexus (CP). Single cells were FACS index sorted followed by Smart-seq2 library preparation and Illumina Nextseq (sequence depth > 1 million per cell). All 1816 cells were grouped into 15 clusters using Seurat package (Macosko, Basu, Satija et al. Cell. 2015), and manually annotated based on gene expression signatures and meta data. We found that the majority of adult microglia expressing homeostatic genes are remarkably similar in transcriptomes, regardless of brain region. By contrast, postnatal microglia represent a more heterogeneous population. We discovered a proliferative region-associated microglia (PAM) subset, mainly found in developing white matter, that share a characteristic gene signature with degenerative disease-associated microglia (DAM). Such PAM have amoeboid morphology, are metabolically active, and phagocytose newly formed oligodendrocytes. This scRNA-seq atlas will be a valuable resource for dissecting innate immune functions in health and disease.

2018-12-31 | GSE123025 | GEO

Intra-tumor heterogeneity of MLH1 promoter methylation revealed by deep single molecule bisulfite sequencing.

Project description: Not available

| S-EPMC2724279 | biostudies-literature

Single-cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors.

Project description: Not available

| S-EPMC5730887 | biostudies-literature

Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites.

Project description: Not available

| S-EPMC7900125 | biostudies-literature

Single-cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors

Project description:This SuperSeries is composed of the SubSeries listed below.