Transcriptomics,Genomics

Dataset Information

35

Targets of JAK STAT signaling in Drosophila cells


ABSTRACT: Purpose: analyze ligand and receptor genes regulated at early time points following JAK STAT pathway stimulation During development and homeostasis, cells integrate multiple signals originating either from neighboring cells or systemically. In turn, responding cells can produce signals that act in an autocrine, paracrine, or endocrine manner. Although the nature of the signals and pathways used in cell-cell communication are well characterized, we lack, in most cases, an integrative view of signaling describing the spatial and temporal interactions between pathways (e.g., whether the signals are processed sequentially or concomitantly when two pathways are required for a specific outcome). To address the extent of cross-talk between the major metazoan signaling pathways, we characterized immediate transcriptional responses to either single- or multiple pathway stimulations in homogeneous Drosophila cell lines. Our study, focusing on seven core pathways, epidermal growth factor receptor (EGFR), bone morphogenetic protein (BMP), Jun kinase (JNK), JAK/STAT, Notch, Insulin, and Wnt, revealed that many ligands and receptors are primary targets of signaling pathways, highlighting that transcriptional regulation of genes encoding pathway components is a major level of signaling cross-talk. In addition, we found that ligands and receptors can integrate multiple pathway activities and adjust their transcriptional responses accordingly. RNA-seq data is for JAK STAT pathway only Overall design: S2R+ or STAT92E mutant cells were treated with upd ligand or control media for 1 hour before harvesting RNA and sequencing

INSTRUMENT(S): Illumina NextSeq 500 (Drosophila melanogaster)

SUBMITTER: Yanhui Hu  

PROVIDER: GSE123167 | GEO | 2018-12-01

REPOSITORIES: GEO

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Publications

Mapping signaling pathway cross-talk in Drosophila cells.

Ammeux Noemie N   Housden Benjamin E BE   Georgiadis Andrew A   Hu Yanhui Y   Perrimon Norbert N  

Proceedings of the National Academy of Sciences of the United States of America 20160815 35


During development and homeostasis, cells integrate multiple signals originating either from neighboring cells or systemically. In turn, responding cells can produce signals that act in an autocrine, paracrine, or endocrine manner. Although the nature of the signals and pathways used in cell-cell communication are well characterized, we lack, in most cases, an integrative view of signaling describing the spatial and temporal interactions between pathways (e.g., whether the signals are processed  ...[more]

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