VEGAS: A Platform for Continuous Directed Evolution in Mammalian Cells (RNAseq)

ABSTRACT: Sindbis virus is a mutagenic ssRNA arbor virus. The structural genome component of this virus can be exchanged for any transgene of < 6kb in size. Here we sequence transgenic elements inserted to the virus. Our EGFP transgene was analyzed as a function of time during viral replication to quantify the mutagenic rate of the virus. Our nanobody transgene was analyzed to assess cDNA library complexity and identity.

ORGANISM(S): Sindbis virus

PROVIDER: GSE123269 | GEO | 2018/12/04

REPOSITORIES: GEO