Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-sequencing and CRISPR/Cas9 Screen of PEO1 cells grown in suspension.

ABSTRACT: RNA-sequencing of PEO1 cells grown in suspension (SUS1 and SUS3) was performed to evaluate transcriptional reprogramming during anokis escape. CRISPR/Cas9 screen of PEO1 cells grown in adherent and suspension culture settings for 5 days or 10 days. GeCKO v2 library transduced in PEO1 cell line and cells were selected with puromycin. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G).

ORGANISM(S): Homo sapiens

PROVIDER: GSE123290 | GEO | 2018/12/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptomic analyssis following EHMT1/2 inhibition

Project description:RNA was isolated from PEO1 olaparib resistant (PEO1-OR) cells grown in complete growth media or PEO1-OR clone 4 cells grown in complete growth media with 500 nanomolar of UNC0642 for 72 hrs. RNA-sequencing was performed to evaluate UNC0642-dependent transcriptional reprogramming, which contributes to resensitization of PEO1-OR to olaparib. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G).

2019-08-16 | GSE135864 | GEO

Activation of Wnt signaling promotes olaparib resistant ovarian cancer.

Project description:Sequencing of olaparib-resistant PEO1 derivatives (C4, C5, C10 and C18) and parental PEO1 (P1 and P2) cells was performed in order to determine mechanisms of acquired resistance in the resistant cell lines. PEO1 parental cell lines were authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G). Olaparib PEO1 resistant cells were generated through a step-wise escalation of olaparib (10nM to 8uM olaparib). In olaparib resistant lines an increase canonical Wnt signaling and loss of of non-canonical Wnt signaling was observed.

2018-07-28 | GSE117765 | GEO

RNA-sequencing and CRISPR/Cas9 Screen of PEO1 cells grown in suspension.

Project description:RNA-sequencing and CRISPR/Cas9 Screen of PEO1 cells grown in suspension.

| PRJNA508193 | ENA

Hindlimb suspension and reloading study

Project description:Analysis of effect of hindlimb suspension and reloading on C57Bl/6 mouse soleus muscle. Experimental groups examined: -Control mice, 14 days -Hindlimb suspension for 7 days -Hindlimb suspension for 7 days, and subsequent reloading for 1 day -Hindlimb suspension for 7 days, and subsequent reloading for 7 days Keywords: other

2004-09-30 | GSE1293 | GEO

Hindlimb suspension and reloading study

2010-06-05 | E-GEOD-1293 | biostudies-arrayexpress

scRNA-seq of pluripotent stem cell derived human intestinal organoids grown in suspension culture

Project description:Here, we used single-cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human intestinal organoids (HIOs) grown in suspension culture after 28 days of in vitro growth. Grown in minigut media supplemented with EGF.

2022-02-04 | E-MTAB-11347 | biostudies-arrayexpress

Environmental bacteria suspension

Project description:Environmental bacteria suspension Raw sequence reads

| PRJNA705060 | ENA

RNA-seq of suspension-grown vs. adherent Neisseria gonorrhoeae under conditions of zinc sequestration or zinc excess

Project description:Deep sequencing of cDNA of Neisseria gonorrhoeae bacteria that were grown in suspension or adherent to acid-washed glass coverslips, in media where zinc was sequestered or available in excess.

2022-12-12 | GSE191020 | GEO

Gene expression and survival changes in Saccharomyces cerevisiae during suspension culture.

Project description:This study explores the connection between changes in gene expression and the genes that determine strain survival during suspension culture, using the model eukaryotic organism, Saccharomyces cerevisiae. The Saccharomyces cerevisiae homozygous diploid deletion pool, and the BY4743 parental strain were grown for 18 hours in a rotating wall vessel, a suspension culture device optimized to minimize the delivered shear. In addition to the reduced shear conditions, the rotating wall vessels were also placed in a static position or in a shaker in order to change the amount of shear stress on the cells. Keywords: shear stress, time course

2006-06-15 | GSE2818 | GEO

Biofilm and BES suspension

Project description:Biofilm and BES suspension Genome sequencing

| PRJNA686243 | ENA