Genomics

Dataset Information

0

Transcriptomic analysis of the Xrn1 depletion by Auxin-degron shutoff


ABSTRACT: The goal of the project was to study the effects on transcription and mRNA stability of the Xrn1 sudden depletion. We analyzed the effect of Xrn1 depletion caused by protein degradation of an Auxin-degron fusion on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 30 min after Auxin addition with a control at 0 min. Overall design: Cells were grown in YPD at 30ºC to 0.5 OD600. At 0 min and 30 min after Auxin addition cells were spun-down and collected. Please note that each sample record represents three replicates (more details in the sample description field).

INSTRUMENT(S): Valencia yeast v12

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jose E. Perez-Ortin  

PROVIDER: GSE123326 | GEO | 2019-02-22

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE123326_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 2 of 2
altmetric image

Publications


The highly conserved 5'-3' exonuclease Xrn1 regulates gene expression in eukaryotes by coupling nuclear DNA transcription to cytosolic mRNA decay. By integrating transcriptome-wide analyses of translation with biochemical and functional studies, we demonstrate an unanticipated regulatory role of Xrn1 in protein synthesis. Xrn1 promotes translation of a specific group of transcripts encoding membrane proteins. Xrn1-dependence for translation is linked to poor structural RNA contexts for translati  ...[more]

Similar Datasets

2019-02-08 | GSE109734 | GEO
| PRJNA508323 | ENA
2015-01-01 | S-EPMC4826345 | BioStudies
2019-08-21 | GSE127875 | GEO
2018-01-01 | S-EPMC6070002 | BioStudies
2019-01-01 | S-EPMC6428865 | BioStudies
2019-01-01 | S-EPMC6765136 | BioStudies
2015-01-01 | S-EPMC4374687 | BioStudies
2018-01-01 | S-EPMC5766927 | BioStudies
2011-01-01 | S-EPMC3090138 | BioStudies