Similar Datasets
Project description:Single-cell mRNA sequencing (scRNA-seq) study was conducted to to probe heterogeneity of human BPH associated leukocytes
2022-02-16 | GSE164695 | GEO
Project description:Single-cell mRNA sequencing (scRNA-seq) study was conducted to to probe heterogeneity of human BPH associated cells. No cell sorting was conducted so all cells within the BPH environment could be represented.
2022-02-16 | GSE183676 | GEO
Project description:Cervical cancer (CC) is the fourth leading cause of deaths in gynecological malignancies. Although the etiology of CC has been extensively investigated, the exact pathogenesis of CC remains incomplete. Recently, single-cell technologies demonstrated advantages in exploring intra-tumoral diversification among various tumor cells. However, single-cell transcriptome (scRNA-seq) analysis of CC cells and microenvironment has not been conducted. In this study, a total of 6 samples (3 CC and 3 adjacent normal tissues) were examined by scRNA-seq. Here, we performed single-cell RNA sequencing (scRNA-seq) to survey the transcriptomes of 57,669 cells derived from three CC tumors with paired normal adjacent non-tumor (NAT) samples. Single-cell transcriptomics analysis revealed extensive heterogeneity in malignant cells of human CCs, wherein epithelial subpopulation exhibited different genomic and transcriptomic signatures. We also identified cancer-associated fibroblasts (CAF) that may promote tumor progression of CC, and further distinguished inflammatory CAF (iCAF) and myofibroblastic CAF (myCAF). CD8+ T cell diversity revealed both proliferative (MKI67+) and non-cycling exhausted (PDCD1+) subpopulations at the end of the trajectory path. We used the epithelial signature genes derived from scRNA-seq to deconvolute bulk RNA-seq data of CC, identifying four different CC subtypes, namely hypoxia (S-H subtype), proliferation (S-P subtype), differentiation (S-D subtype), and immunoactive (S-I subtype) subtype. Our results lay the foundation for precision prognostic and therapeutic stratification of CC.
2022-10-04 | E-MTAB-11948 | biostudies-arrayexpress
Project description:Contextual cues from cancer cells govern CAF heterogeneity [scRNA-seq]
| PRJNA701029 | ENA
Project description:Chromatin assembly factor CAF-1 is conserved in all eukaryotes. In contrast to animals, plant CAF-1 is viable. Here we probe effects of loss of CAF-1 on the transcriptome of non-diving cells. Fully expanded rosette leaf 6 was collected from 10 35-d-old plants grown in short day photoperiods of wild type Col and CAF-1 mutants fas2-4 were used for RNA extraction.
2015-11-02 | E-MTAB-2376 | biostudies-arrayexpress
Project description:Chromatin assembly factor CAF-1 is conserved in all eukaryotes. In contrast to animals, plant CAF-1 is viable. Here we probe effects of loss of CAF-1 on the transcriptome of non-diving cells. Fully expanded rosette leaf 6 was collected from 10 35-d-old plants grown in short day photoperiods of wild type Col and CAF-1 mutants fas2-4 generation 1 and generation 6 were used for RNA extraction.
2018-08-31 | E-MTAB-6136 | biostudies-arrayexpress
Project description:Purpose : Primary cultured fibroblast cultures can be classified based on their tumor promoting ability. Normal associated fibroblasts (NAF) cannot transform benign epithelia. Cancer associated fibroblasts (CAF) can transform benign epithelia into cancer. As described in Kato and Placencio et al., High passage CAF (CAF_HiP) cells have lost the ability to potentiate tumor growth. This study sought to identify the differences among three groups of fibroblasts: NAF, CAF, and CAF_HiP Methods :Human male prostatectomy tissues were cultured to generate primary cultured fibroblast cultures. Results and Conclusions : Differential gene expression among the top 200 genes analzed as described in Kato and Placencio et al. revealed 33 secreted proteins. SFRP1 was confirmed to be highly expressed in CAF and was shown to play a role in prostate cancer tumor progression and neuroendocrine differentiation.
2019-06-01 | GSE99744 | GEO
Project description:Compare the difference between pairwise aNOF and CAF samples for two patients patient #225: aNOF #225 vs CAF #225 patient #248: aNOF #248 vs CAF #248
2012-01-27 | E-GEOD-35249 | biostudies-arrayexpress
Project description:Gene expression profiling of CAF co-cultured with human oral squamous cell carcinoma (OSCC) cells compared with mono-cultured CAF. To identify key molecular regulators expressed by carcinoma-associated fibroblasts (CAF) that promote cancer cell invasion, microarrays were performed by comparing co-cultured OSCC cells and CAF with monoculture controls. comparison 1: CAF co-cultured with OSCC vs. mono-cultured CAF comparison 2: OSCC co-cultured with CAF vs. mono-cultured OSCC 1.7X10(5) YD-10B OSCC cells and 1.7X10(5) CAF were seeded in the upper chamber and lower chamber, respectively, of 6-transwell plates containing collagen-coated 1 micrometer pore transmembrane filters (Becton Dickinson, Franklin Lakes, NJ, USA). Monoculture control samples were generated by culturing only CAF or OSCC on the same side of the filter as in the co-culture design.
2010-06-21 | E-GEOD-18532 | biostudies-arrayexpress