Randomized PAM depletion of AsCas12a

ABSTRACT: We report the PAMs of AsCas12a using a cell-free TXTL-based cleavage assay. By adding randomized PAM library and AsCas12a-gRNA in vitro, functional PAM sequences were cleaved, while non-functional PAMs remained. By amplifying the non-cleaved DNA, we use next-generation sequencing to analyze the depletion of functional PAMs of AsCas12a.

ORGANISM(S): synthetic construct

PROVIDER: GSE123443 | GEO | 2019/01/01

REPOSITORIES: GEO