Genomics

Dataset Information

24

Unbiased screen of RNA tailing enzymes reveals a poly(UG) polymerase required for genome integrity and RNA silencing


ABSTRACT: Ribonucleotidyl transferases (rNTases) add non-templated ribonucleotides to diverse RNAs. We developed a screening strategy in S. cerevisiae to identify sequences added by candidate enzymes from different organisms at single-nucleotide resolution. The rNTase activities of 19 previously unexplored enzymes were determined. In addition to poly(A)- and poly(U)-adding enzymes, we identified a C-adding enzyme that is likely part of a two-enzyme system that adds CCA to tRNAs in a eukaryote; a nucleotidyl transferase that adds nucleotides to RNA without apparent nucleotide preference; and a poly(UG) polymerase, C. elegans MUT-2, which adds alternating U and G nucleotides to form poly(UG) tails. MUT-2 is known to be required for certain forms of RNA silencing, and mutations in the enzyme that are defective in silencing also fail to add poly(UG) tails in our assay. We propose that MUT-2 poly(UG) polymerase activity is required to promote genome integrity and RNA silencing. Overall design: We developed a screening approach to identify enzymes that add non-templated nucleotides to RNAs. Candidate rNTases were tethered in vivo to a reporter RNA in S. cerevisiae, and the number and identity of nucleotides they added were determined at single-nucleotide resolution using high-throughput sequencing.

INSTRUMENT(S): Illumina HiSeq 2000 (Saccharomyces cerevisiae BY4741)

SUBMITTER: Melanie Preston 

PROVIDER: GSE123478 | GEO | 2018-12-08

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE123478_Preston_et_al_2018-TailCompSummaryForAllSamples-paper_figs.xlsx.gz Xlsx
GSE123478_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 3 of 3

Similar Datasets

2019-01-01 | S-EPMC6613791 | BioStudies
2020-05-12 | GSE146618 | GEO
2020-05-12 | GSE148074 | GEO
2016-01-01 | S-EPMC5009732 | BioStudies
2012-01-01 | S-EPMC3766730 | BioStudies
2008-01-01 | S-EPMC2676681 | BioStudies
2020-05-12 | GSE148133 | GEO
2011-11-17 | E-GEOD-33756 | ArrayExpress
1000-01-01 | S-EPMC3985877 | BioStudies
2016-01-01 | S-EPMC4931116 | BioStudies