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Microglia in developing retina transition through a disease-like functional state that does not require CSF1R signaling for survival

ABSTRACT: Microglia have important remodeling functions in development and disease. There is evidence for molecular diversity of microglia suggesting they may exist in distinct functional states to differentially impact CNS health and function. To better understand this in development, we profiled microglia of a discrete developing CNS region, the murine retina. We find that retinal microglia transition through unique transcriptional states and identify a population with peak density postnatally that resemble adult disease-associated microglia (DAM) and CD11c+ microglia of developing white matter, we term DAM-like. Developmental cell death is a major driver of the DAM-like phenotype, and TREM2 signaling is required for select DAM gene expression. Notably, DAM-like cells that highly express CD11c are not dependent on CSF1R signaling for survival, and TREM2 signaling is required for CSF1R independence in a subset of microglia. Thus, microglial phenotype in development is influenced by local developmental events and may share features with microglia in disease.

ORGANISM(S): Mus musculus

PROVIDER: GSE123757 | GEO | 2019/06/10

REPOSITORIES: GEO

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TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration

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2022-08-12 | PXD029429 | Pride

Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and -independent cellular responses in Alzheimer’s disease [7 months]

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Human and mouse single-nucleus transcriptomics reveal TREM2-dependent and -independent cellular responses in Alzheimer’s disease [15 months]

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Single cell RNA-seq identifies a unique microglia type associated with Alzheimer’s disease [RNA]

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Cortical single-cell RNA sequencing of the microglia specific TREM2 inducible mouse models in the middle stage of amyloid deposition

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Cortical single-cell RNA sequencing of the microglia specific TREM2 inducible mouse models in the early stage of amyloid deposition

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2022-04-27 | GSE192600 | GEO

Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [RNA-Seq]

2022-04-27 | GSE192601 | GEO

Dap12 microglia

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