Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Yeast sirutin family members maintain trannscription homeostasis to ensure genome stability

ABSTRACT: Loss of Hst3 and Hst4 leads to increased nascent transcription which increases the prevelance of R-loops and contributes to DNA damage phenotype of the mutant

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE124132 | GEO | 2019/05/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Yeast Sirtuin Family Members Maintain Transcription Homeostasis to Ensure Genome Stability.

Project description: Not available

| S-EPMC6640630 | biostudies-literature

Yeast sirutin family members maintain trannscription homeostasis to ensure genome stability

Project description:Yeast sirutin family members maintain trannscription homeostasis to ensure genome stability

| PRJNA510821 | ENA

Sir2-family of histone deacetylase regulated gene expression in Candida glabrata

Project description:Sir2-family of NAD-dependent histone deacetylases are involved in gene transcription repression in yeast. The C. glabrata genes encoding members of this family are SIR2 (CAGL0K01463g), HST1 (CAGL0C05357g), HST2 (CAGL0L08668g), HST3 (CAGL0H08239g) and HST4 (CAGL0F05621g). SIR4 (CAGL0K11396g) encodes a factor that forms a functional complex with Sir2. In this experiment, the transcription profile of C. glabrata HST1 null, HST2 null, SIR2 null or SIR4 null strain individually comparing with that of wild type strain was determined. All strains were grown in YPD liquid medium in Log phase at 30 C. Keywords: transcriptional profiling by microarray

2007-01-04 | GSE6626 | GEO

Expression analysis of the Saccharomyces cerevisiae hst3hst4 mutant

2008-12-04 | GSE13766 | GEO

Transcription profiling of S. cerevisiae hst3hst4 mutant

Project description:The hst3hst4 strain (FY background) has the HST3 and HST4 genes, encoding putative NAD-dependent deacetylases that regulate histone 3 K56 acetylation, deleted. Expression profiling using Affymetrix microarrays was used to assess the change in the gene expression in this strain in comparison to wild-type under normal growth conditions. Experiment Overall Design: Total RNA was extracted from two independent cultures of the hst3hst4 strain and the wt strain, and was hybridized to microarrays. Gene expression levels of each mutant culture was compared to each wt culture.

2009-01-13 | E-GEOD-13766 | biostudies-arrayexpress

ALBA protein complex reads genic R-loops to maintain genome stability in Arabidopsis

Project description:The R-loop, composed of a DNA-RNA hybrid and the displaced single-stranded DNA, regulates diverse cellular processes. However, how cellular R-loops are sensed remain poorly understood. Here we report the discovery of the evolutionally conserved ALBA proteins (AtALBA1&2) functioning as the R-loops sensor of genic regions in higher plant Arabidopsis thaliana. AtALBA1 and 2 form a heterodimer protein complex and localize in the nucleus. While AtALBA1 binds to the DNA-RNA hybrid strand, AtALBA2 associates with single-stranded DNA in the R-loops. AtALBA1&2 preferentially binds to the genic R-loop regions that are associated with active epigenetic marks. Depletion of AtALBA1 or AtALBA2 results in hypersensitivity of plants to DNA damaging agents. Our results demonstrate the AtALBA1&2 protein complex is the Arabidopsis genic R-loops sensor for maintaining genome stability in Arabidopsis.

2019-04-17 | GSE121683 | GEO

Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [TT-seq]

Project description:Connections between RNA polymerase II (RNAPII) transcription stress, R-loops, and genome instability have been established however, the underlying mechanisms remain poorly understood. Here we used a mutant version of elongation factor TFIIS (TFIISmut) to specifically induce increased levels of RNAPII pausing, arrest, and/or backtracking in human cells. TFIISmut expression results in slower elongation rates, relative depletion of polymerases from the end of genes, and increased levels of stopped RNAPII. It affects mRNA splicing and termination as well. Remarkably, however, TFIISmut expression also dramatically increases R-loops, which may form at the anterior end of backtracked RNAPII and trigger genome instability, including DNA strand breaks. These results shed new light on the relationship between transcription stress and R-loops, and suggest that different classes of R-loops exist, potentially with distinct consequences for genome instability.

2019-06-13 | GSE132395 | GEO

Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [ChIP-seq]

2019-06-13 | GSE132391 | GEO

Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [ RNA-seq]

2019-06-13 | GSE132398 | GEO

Regulation of copper homeostasis by members of the COMMD protein family.

Project description: Not available

| S-EPMC7803461 | biostudies-literature