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FGFR2b/ßcatenin signalling pathway in embryonic mouse lungs

ABSTRACT: FGFR2b signalling interacts with ßcatenin signalling to regulate early branching morphogenesis. Earlier, we identified a set of genes regulated in the epithelium of the developing lung by the global inhibition of FGFR2b signalling after 6 and 9 hours, in vivo. In this experiment, we used a pharmacological inhibitor of ßcatenin signalling, IQ1, to block ßcatenin signalling in isolated E12.5 wildtype lungs for 9 and 24 hrs, in vitro. We intend to assess the overlap between genes regulated in our in vivo experiment with those regulated in our in vitro, IQ1 experiment. In so doing, we hope to identify genes regulated by the FGFR2b/ßcatenin signalling pathway. Samples were obtained by isolating E12.5 lungs from C57bl/6J mice. Isolated lungs were then cultured on filters with culture medium supplied with 10 uM IQ1 (experimental) or vehicle (control), for 9 and 24 hrs. After these times, the experiments were stopped, and samples were prepared for RNA isolation.

ORGANISM(S): Mus musculus

PROVIDER: GSE124155 | GEO | 2019/01/25

REPOSITORIES: GEO

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