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Transcriptional profile of Sacchromyces cerevisiae in different levels of oxygen provision

ABSTRACT: Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 0%, 0.5%, 1.0%, 2.8% or 20.9% O2 in the inlet gas (D= 0.10 /h, pH5, 30C).

ORGANISM(S): Saccharomyces cerevisiae Schizosaccharomyces pombe

PROVIDER: GSE12442 | GEO | 2009/10/05

SECONDARY ACCESSION(S): PRJNA112995

REPOSITORIES: GEO

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Transcription profiling of yeast grown in different levels of oxygen provision

Project description:Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 0%, 0.5%, 1.0%, 2.8% or 20.9% O2 in the inlet gas (D= 0.10 /h, pH5, 30C). Experiment Overall Design: Four (0,0.5,2.8 and 20.9% oxygen) or seven samples (1% oxygen) were analysed. Samples were either from four (0,1,20.9% oxygen) or two independent cultivations (0.5, 2.8% oxygen). One sample (VTT_Ys23_13_1%) was found to be an outlier and was removed from the analysis.

2009-10-10 | E-GEOD-12442 | biostudies-arrayexpress

Transcriptional response of Sacchromyces cerevisiae to change in oxygen provision

Project description:In industrial fermentations of Saccharomyces cerevisiae, transient changes in oxygen concentration commonly occur and it is important to understand the behaviour of cells during these changes. Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 1.0% and 20.9% O2 in the inlet gas (D= 0.10 /h, pH5, 30C). After steady state was achieved, oxygen was replaced with nitrogen and cultures were followed until new steady state was achieved. The overall responses to anaerobic conditions of cells initially in different conditions were very similar. Independent of initial culture conditions, transient downregulation of genes related to growth and cell proliferation, mitochondrial translation and protein import, and sulphate assimilation was seen. In addition, transient or permanent upregulation of genes related to protein degradation, and phosphate and amino acid uptake was observed in all cultures. However, only in the initially oxygen-limited cultures was a transient upregulation of genes related to fatty acid oxidation, peroxisomal biogenesis, oxidative phosphorylation, TCA cycle, response to oxidative stress, and pentose phosphate pathway observed. Furthermore, from the initially oxygen-limited conditions, a rapid response around the metabolites of upper glycolysis and the pentose phosphate pathway was seen, while from the initially fully aerobic conditions, a slower response around the pathways for utilisation of respiratory carbon sources was observed.

2011-04-01 | GSE22832 | GEO

Transcriptional response of Sacchromyces cerevisiae to change in oxygen provision

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RNA sequencing experiment of S. Cerevisiae strain CEN.PK113-7D in four different chemostat conditions

Project description:The RNA sequencing experiment was performed in four different chemostat conditions as control for a CAGE sequencing experiment performed in the same conditions using the industrial relevant S. Cerevisiae strain CEN.PK113-7D

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Project description:Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present. We used microarrays to detail the hypoxia upregulated gene in human primary macrophages. Experiment Overall Design: Primary macrophages were differentiated for 7 days in vitro from human peripheral blood (monocyte-derived macrophages, MDMs) and were subjected to severe hypoxia (< 0.5% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators.

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Transcription profiling of yeast grown in fermentors at different dilution rates

Project description:the protrotophic laboratory strain CEN.PK113-7D (MAT a) was grown in laboratory fermentors with a working volume of 1 litre at dilution rates of 0.02, 0.05, 0.10 (in triplicate), 0.20 (in triplicate), 0.25, and 0.33 per hour (in triplicate). At steady state, samples from each of the 12 continuous cultures were taken and cooled below 2 degree C within ten seconds by mixing 50\% sample and 50\% crushed ice.

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Glioma cells oxygen response

Project description:Transcriptomic analyses of the oxygen response of two glioma cell lines at 20% versus 0.3% O2, and 3% vs 0.3% O2 in the presence or absence of serum

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Project description:The protrotophic laboratory strain CEN.PK113-7D (MAT a) and three knock-out strains snf1, snf4 and snf1snf4 were grown in laboratory fermentors with a working volume of 1 litre at dilution rate (D) of 0.10 per hour (in triplicate for each strain). At steady state, samples from each of the 12 continuous cultures were taken and cooled below 2 degree C within ten seconds by mixing 40% sample and 60% crushed ice.

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