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Liver-specific overexpression of GBP5 induces liver injury and inflammation

ABSTRACT: Purpose: The goal of this study is to compare transcriptome profilings of livers in which GBP5 and βGal were overexpressed, respectively. Methods: Liver-specific GBP5-overexpressing and βGal–overexpressing mice were generated by tail-vein injection of individual purified adenovirus(6 × 1011 viral particles per mouse), respectively. RNA-seq was performed by using HiSeq X Ten platform in a single test. Paired-end clean reads were aligned to the mouse reference genome (Ensemble_GRCm38.89) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1). Conclusion: Our study represents the first detailed analysis of liver transcriptomes from GBP5- and βGal-overexpressing livers, generated by RNA-seq technology. Our results show that 633 genes were significantly up-regulated in the GBP5-overexpressing livers, whereas 586 genes were dramatically down-regulated in the GBP5-overexpressing livers. GO analysis showed that some up-regulated genes were related to the immune system process, response to stress, cell cycle, and cell death in GBP5-overexpressing livers.

ORGANISM(S): Mus musculus

PROVIDER: GSE124520 | GEO | 2021/12/29

REPOSITORIES: GEO

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D-galactosamine (GalN) and lipopolysaccharide (LPS) effect on GBP5 deficient liver

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2021-12-28 | GSE124473 | GEO

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2019-09-23 | GSE136728 | GEO

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2023-06-15 | GSE152542 | GEO

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2020-07-28 | GSE130121 | GEO

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2022-06-02 | GSE168850 | GEO

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2018-12-30 | GSE112511 | GEO

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2020-02-06 | GSE133216 | GEO

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Title: RNA-seq analysis of mRNA profiles in the livers of HuR flox/flox and hepatocyte-specific HuR knockout (HKO) mice fed with an MCD for 3 weeks.

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2023-06-17 | GSE152670 | GEO

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