Transcriptomics,Genomics

Dataset Information

48

Screen of oligonucleotides for microarray transcriptome analysis of Populus deltoides and P. trichocarpa


ABSTRACT: The purpose of this study was to evaluate a set of 6-7 long oligonucleotide probes developed based on the sequence of the Populus trichocarpa genome, that are optimal for gene expression analysis of P. deltoides and a hybrid of P. deltoides and P. trichocarpa. To evaluate these probes, multiple tissues (differentiating xyle, leaf and whole-root) of a pure P. deltoides and a hybrid (P. deltoides X P. trichocarpa) were transcript profiled for identification of one or more probes that are not biased towards one or the other genotype. Overall design: Three vegetative tissues (root, leaf and xylem) harvested from two biological replicates from each genotypes of Populus deltoides (genotype D124) and a Populus hybrid (P. deltoides X P. trichocarpa, genotype 52-225) were hybridized separately to microarrays in a single-dye experiment.

INSTRUMENT(S): Populus trichocarpa microarray

SUBMITTER: Matias Kirst  

PROVIDER: GSE12492 | GEO | 2008-09-10

SECONDARY ACCESSION(S): PRJNA112971

REPOSITORIES: GEO

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