Transcriptomics,Genomics

Dataset Information

48

A TGFB Responsive Gene Expression Signature is Associated With More Severe Skin and Lung Disease in Diffuse Scleroderma


ABSTRACT: Primary dermal fibroblasts from patients with dSSc and healthy controls were treated with TGF beta for up to 24h and the genome-wide patterns of gene expression measured on DNA microarrays. 894 genes were identified as TGF beta-responsive in 4 independent cultures of dermal fibroblasts (2 healthy control and 2 dSSc patients). The 894 genes in the TGF beta-responsive signature are associated with induction of growth factor signaling, collagen synthesis and extracellular matrix deposition. Overall design: Common reference design; we used Universal Human Reference RNA (Stratagene) as our reference for every array; For every hybridization sample RNA was always labeled with Cy3, and reference RNA was always labeled with Cy5 All processed data is displayed as log2 of the LOWESS-normalized Cy5/Cy3 ratio and was multiplied by negative 1 for analysis in the associated publication.

INSTRUMENT(S): Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)

SUBMITTER: Jennifer L Sargent  

PROVIDER: GSE12493 | GEO | 2009-09-15

SECONDARY ACCESSION(S): PRJNA112973

REPOSITORIES: GEO

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Publications

A TGFbeta-responsive gene signature is associated with a subset of diffuse scleroderma with increased disease severity.

Sargent Jennifer L JL   Milano Ausra A   Bhattacharyya Swati S   Varga John J   Connolly M Kari MK   Chang Howard Y HY   Whitfield Michael L ML  

The Journal of investigative dermatology 20091008 3


Systemic sclerosis is a complex disease with widespread skin fibrosis and variable visceral organ involvement. Since transforming growth factor-beta (TGFbeta) has been implicated in driving fibrosis in systemic sclerosis, a mechanism-derived gene expression signature was used to assay TGFbeta-responsive gene expression in the skin of patients with systemic sclerosis (SSc). Primary dermal fibroblasts from patients with diffuse SSc (dSSc) and healthy controls were treated with TGFbeta, and the gen  ...[more]

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