Method for absolute quantification of microbial communities by using both microarrays and competitive PCR

ABSTRACT: We investigated an improved method that combines competitive PCR and microarray techniques. This approach allowed us to quantify specific bacterial groups mounted on DNA chips with accuracy close to that of real-time PCR, despite a measurement at the end point of PCR, and also to estimate the bacterial DNA content in sample DNA.

ORGANISM(S): Campylobacter rectus Prevotella intermedia Acinetobacter baumannii Treponema denticola Streptococcus intermedius Streptococcus gordonii Streptococcus agalactiae Pseudomonas aeruginosa Streptococcus mutans Staphylococcus epidermidis Cereibacter sphaeroides Bacillus cereus Prevotella nigrescens Cutibacterium acnes Porphyromonas gingivalis Tannerella forsythia Escherichia coli Fusobacterium nucleatum Lactobacillus gasseri Enterococcus faecalis Homo sapiens Capnocytophaga gingivalis Neisseria meningitidis Bacteria Helicobacter pylori Phocaeicola vulgatus Bifidobacterium adolescentis Aggregatibacter actinomycetemcomitans Schaalia odontolytica Staphylococcus aureus Deinococcus radiodurans Clostridium beijerinckii Fusobacterium nucleatum subsp. nucleatum

PROVIDER: GSE125085 | GEO | 2019/01/16

REPOSITORIES: GEO