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Small RNA from S2 cell infected with FHV deltaB2 particle

ABSTRACT: S2 cells were infected with FHV deltaB2 particle and the S2 cells were harvested to isolate total RNA at 96 hpi. Small RNAs were separated on a denaturing 15% polyacrylamide gel. Ligation to adapters requires 5' monophosphate and 3' OH. Note: The base quality (.qual) file corresponding to the fasta format (.fna) file linked to Sample GSM306489 is unavailable.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE12527 | GEO | 2008/10/16

SECONDARY ACCESSION(S): PRJNA112933

REPOSITORIES: GEO

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Small RNA from S2 cell infected with FHV deltaB2 particle

Project description:S2 cells were infected with FHV deltaB2 particle and the S2 cells were harvested to isolate total RNA at 96 hpi. Small RNAs were separated on a denaturing 15% polyacrylamide gel. Ligation to adapters requires 5' monophosphate and 3' OH. Note: The base quality (.qual) file corresponding to the fasta format (.fna) file linked to Sample GSM306489 is unavailable. Small RNAs were sequenced from S2 cell after virus infection.

2008-10-15 | E-GEOD-12527 | biostudies-arrayexpress

Transcriptional response of Drosophila cells to FHV infection (infection experiment)

2009-04-01 | GSE15466 | GEO

Transcriptional response of Drosophila cells to FHV RNA1 replicon expression (replicon experiment)

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Transcriptional response of Drosophila cells to FHV infection (infection experiment)

Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to infection with Flock House virus (FHV). Experiment Overall Design: Cultured S2 cells were infected with FHV at an MOI of 10 and we measured global transcript levels at 12 h after infection compared to control mock infected cells using Affymetrix Drosophila Genome 1.0 microarray chips.

2009-04-11 | E-GEOD-15466 | biostudies-arrayexpress

Transcription profiling of Drosophila cells to FHV RNA1 replicon expression (replicon experiment)

Project description:Virus infections induce cellular gene up and down regulation, and these changes often provide clues to cellular pathways utilized by viruses. We used microarrays to examine the transcriptional responses of cultured Drosophila S2 cells to Flock House virus (FHV) replicon induction. Experiment Overall Design: Cultured S2 cells stably transfected with either a control replicon (pS2F1fs) or an FHV RNA1 replicon (pS2F1) were induced with 1 mM copper and we measured global transcript levels at 18 h after induction using Affymetrix Drosophila Genome 2.0 microarray chips.

2009-04-11 | E-GEOD-15467 | biostudies-arrayexpress

Viral small RNAs from Drosophila melanogaster flies infected with FHVdeltaB2 or FHV.

Project description:We focus our analyses on the description of viral small RNAs (FHVdeltaB2 or FHV) and based on genetic and molecular evidende, classify them and discuss their relevance in antiviral defense. Small RNAs from adult Drosophila flies from different genetic backgrounds and infected with FHV-deltaB2 or FHV were sequenced using the illumina platform.

2011-11-12 | E-GEOD-32011 | biostudies-arrayexpress

Transcription profiling of Drosophila cells response to FHV RNA replication

Project description:This SuperSeries is composed of the following subset Series:; GSE15466: Transcriptional response of Drosophila cells to FHV infection (infection experiment); GSE15467: Transcriptional response of Drosophila cells to FHV RNA1 replicon expression (replicon experiment) Experiment Overall Design: Refer to individual Series

2009-04-10 | E-GEOD-15469 | biostudies-arrayexpress

Viral small RNAs from Drosophila melanogaster flies infected with FHVdeltaB2 or FHV.

2011-11-12 | GSE32011 | GEO

High-throughput sequencing of small RNAs in chicken somite embryos

Project description:High-throughput sequencing of small RNAs in chicken somite embryos Dissected tissue white leghorn chicken embryos was disaggregated and RNA extracted using the miRVana kit (Ambion). Small RNA fraction between 19 and 24 nt was isolated from 15% denaturing polyacrylamide gel and 15 micro gram was ligated to Solexa adaptors (Illumina) without de-phosphorylating and re-phosphorylating. The short RNAs were converted to DNA by RT-PCR following the Illumina protocol.

2009-04-27 | E-GEOD-15513 | biostudies-arrayexpress

A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol

Project description:Streamlined protocol for fluorescence-based PAR-CLIP (fPAR-CLIP) that eliminates the need to use radioactivity. Protocol is based on direct ligation of a fluorescently labeled adapter to the 3’end of crosslinked RNA on immobilized ribonucleoproteins, followed by isolation of the RNA and efficient conversion into cDNA without the previously needed size fractionation on denaturing polyacrylamide gels. These improvements cut the experimentation time from 4-5 to 2 days and increases sensitivity by 10-100-fold.

2020-12-29 | GSE163925 | GEO

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