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Global gene expression analysis of undifferentiated H1 hESC, and hEB cells differentiated with SF BVF2H conditions

ABSTRACT: Global gene expression analysis of human embryoid body (hEB) differentiations during differentiation in serum free conditions in the presence of BMP4, VEGF, and FGF2 growth factors A total of 5 samples are analyzed. Each sample is taken at each time point. There is no replicate. H1_hESC_day0 sample is used as control to compare to other samples Keywords: Cell differentiation

ORGANISM(S): Homo sapiens

PROVIDER: GSE12531 | GEO | 2008/08/23

SECONDARY ACCESSION(S): PRJNA113241

REPOSITORIES: GEO

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Global gene expression analysis of undifferentiated H1 hESC, and hEB cells differentiated with SF BVF2H conditions

Project description:Global gene expression analysis of human embryoid body (hEB) differentiations during differentiation in serum free conditions in the presence of BMP4, VEGF, and FGF2 growth factors A total of 5 samples are analyzed. Each sample is taken at each time point. There is no replicate. H1_hESC_day0 sample is used as control to compare to other samples Keywords: Cell differentiation Cell differentiation compares to undifferentiated status in a time-course of 3-, 5-, 7- and 10-day without replicates.

2008-08-23 | E-GEOD-12531 | biostudies-arrayexpress

Global transcriptome analysis of WT versus HEB-/- hESCs

Project description:To examine genome-wide changes in mRNA expression, we performed RNA-Seq on HEB-/- and WT hESCs. There were 274 significant changes in mRNA expression (p<0.05) between HEB-/- and WT hESCs; 126 transcripts were lower and 148 transcripts were higher

2017-06-24 | GSE100417 | GEO

HEB ChIP-seq in ID4 knockdown Comma-Dβ mammary epithelial cells

Project description:We performed ChIP-seq for the E-protein transcription factor HEB in ID4 knockdown mammary epithelial cells to determine how ID4 affects HEB DNA binding.

2020-05-07 | GSE149968 | GEO

Genes regulated by HEB and E2A during DP stage of thymocyte development

Project description:To investigate gene targets of the E-proteins HEB and E2A during the CD4+CD8+ double positive (DP) stage of T cell development. We examined E-protein function by simultaneous removal of both HEB (Tcf12) and E2A (Tcfe2a) genes at the DP stage. This was done by crossing mice containing HEB floxed and E2A floxed alleles to a CD4Cre background (Tcf12f/fTcfe2af/fCD4Cre mice). Microarray analysis was used to compare gene expression in HEB and E2A double deficient DP thymocytes (Cre+) to Cre- control DP thymocytes. Keywords: genetic modification

2007-12-04 | GSE9749 | GEO

HEB associates with PRC2 and SMAD2/3 to regulate developmental fates [ChIP-Seq]

Project description:We report the interaction between HEB and PRC2 components in mouse embryonic stem cells (ESCs) ChIP-Seq of HEB and SMAD2/3 in mouse ESC and derived endoderm

2015-06-30 | E-GEOD-53233 | biostudies-arrayexpress

Expression data from WT, HEB-KO and E2A-KO LY6D- CLP cells

Project description:The E-protein transcription factors E2A and HEB play important roles at several stages of hematopoiesis. However, the exact mechanism for theire action and the main targets in the LY6D negative common lymphoid progentior (CLP) compartment remains unknown. By adressing this question, we will gain important infromation regarding the early events leading to B-cell specification. FACS sorted LY6D negative common lymphoid progenitors from WT, HEB-KO and E2A-KO mice were subjected to RNA extraction and hybridization on Affymetrix microarrays. At least two independent sorts were performed per genotype. During each sort, cells were pooled from several bone marrows.

2011-11-22 | E-GEOD-27402 | biostudies-arrayexpress

HEB and histone mark ChIP-seq in Comma-Dβ mouse mammary epithelial cells

Project description:The goal of this study was to determine the genome wide binding sites of the E-protein transcription factor HEB in mammary epithelial cells, and to delineate the chromatin context of these regions. ChIP-seq for HEB, H3K4Me3, H3K27Ac, and H3K27Me was performed in Comma-Dβ cells in quadruplicate.

2020-05-07 | GSE149967 | GEO

HEB associates with PRC2 and SMAD2/3 to regulate developmental fates [ChIP-Seq]

Project description:We report the interaction between HEB and PRC2 components in mouse embryonic stem cells (ESCs)

2015-06-30 | GSE53233 | GEO

HEB associates with PRC2 and SMAD2/3 to regulate developmental fates [RNA-Seq]

Project description:We report the interaction between HEB and PRC2 components in mouse embryonic stem cells (ESCs)

2015-06-30 | GSE60285 | GEO

HEB and E2A function as SMAD/FOXH1 cofactors

Project description:Nodal signaling, mediated through SMAD transcription factors, is necessary for pluripotency maintenance and endoderm commitment. We have identified a new motif, termed SMAD Complex Associated (SCA) that is bound by SMAD2/3/4 and FOXH1 in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that two bHLH proteins - HEB and E2A - bind the SCA motif at regions overlapping SMAD2/3 and FOXH1. Further, we show that HEB and E2A associate with SMAD2/3 and FOXH1, suggesting they form a complex at critical target regions. This association is biologically important, as E2A is critical for mesendoderm specification, gastrulation, and Nodal signal transduction in Xenopus tropicalis embryos. Taken together, E2A is a novel Nodal signaling cofactor that associates with SMAD2/3 and FOXH1 and is necessary for mesendoderm differentiation.

2011-08-23 | GSE30146 | GEO

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