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Single cell expression profiling of the mouse Sox17-null endocardium at E8.5

ABSTRACT: To understand the function of Sox17 in the precursor cells of the mouse endocardium and the effect in the develoiping heart tube, single cell expression profiling of the Sox17-null endocardium and myocardium were performed.

ORGANISM(S): Mus musculus

PROVIDER: GSE125321 | GEO | 2019/02/12

REPOSITORIES: GEO

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Single cell expression profiling of the mouse Sox17-null myocardium at E8.5

Project description:To understand the function of Sox17 in the precursor cells of the mouse endocardium amd the effect in the develoiping heart tube, single cell expression profiling of the Sox17-null endocardium and myocardium were performed.

2019-02-12 | GSE125322 | GEO

Single cell expression profiling of the mouse Sox17-null endocardium and myocardium at E8.5

Project description:Single cell expression profiling of the mouse Sox17-null endocardium and myocardium at E8.5

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Single cell expression profiling of the mouse Sox17-null endocardium at E8.5

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Single cell expression profiling of the mouse Sox17-null myocardium at E8.5

Project description:Single cell expression profiling of the mouse Sox17-null myocardium at E8.5

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Single cell expression profiling of the mouse Sox17-null endocardium and myocardium at E8.5

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-02-12 | GSE125323 | GEO

Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice.

Project description: Not available

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Transcriptional regulation of Sox17 in HUVEC

Project description:We identified Sox17 as a novel angiogenic transcription factor in the context of tumor. Our data revealed that Sox17 promotes tumor angiogenesis and tumor vessel abnormality. We found that Sox17 is specifically expressed in tumor endothelial cells within tumors. Our study elucidates a novel transcriptional regulation for tumor angiogenesis Control HUVECs vs. Sox17 knockdown HUVECs. Biological replicates: 3 control replicates, 3 transfected replicates.

2012-12-17 | E-GEOD-37395 | biostudies-arrayexpress

Whole transcriptome analysis of Sox17.Epi and Endo cells in mouse embryos

Project description:This experiment has been done to understand the dynamics of gene expression in Sox17.Epi and Endo cells using multiplex RNA-Seq technology. Sox17.Epi (Sox17^GFP+EpCAM+) and Sox17.Endo (Sox17^GFP+EpCAM-) cells were isolated by FACS from E9.5 mouse embryos. The spatial comparison between Sox17.Epi versus Sox17.Endo provides insight into which genes are differentially of specifically expressed in two populations.

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Sox17-transgenic hematopoietic stem cell microarray

Project description:The transcription factor SOX17 is expressed by fetal, but not adult hematoipoietic stem cells (HSCs), and is required for the maintenance of fetal and neonatal, but not adult, HSCs. In the current study we show that ectopic expression of Sox17 in adult HSCs and transiently reconstituting multipotent progenitors was sufficient to confer increased self-renewal potential and the expression of fetal HSC genes including fetal HSC surface markers. To assess the acute effects of ectopic Sox17 expression on global gene expression in adult HSCs, we performed microarray analysis to compare the gene expression profile of adult Sox17-trangenic and control HSCs after short induction of Sox17-transgene expression. Total RNA were isolated from 5 independent, freshly isolated aliquots of 10,000 HSCs isolated from 8-week old Sox17-transgenic ((tetO)7CMVSox17-IRES-NucEGFP;B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J double transgenic) or littermate control mice that were treated with doxycycline for 5 days to induce transgene expression. Purified RNA was reverse transcribed and amplified using the WT-Ovation™ Pico RNA Amplification system (NuGEN Technologies) following the manufacturer’s instructions. Sense strand cDNA was generated using WT-Ovation™ Exon Module (NuGEN), then fragmented and labeled using the FL-Ovation™ cDNA Biotin Module V2 (NuGEN). 2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.

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Gene expression profile at single cell level of E12.5 mouse heart with transcription factor Gata4 knockout by inducible Cre in endocardium

Project description:Gata4 is required for mouse endocardium development. To identify genes regulated by Gata4 in endocardium, we deleted Gata4 in endocardium with Cdh5(Pac)-CreERT2 and performed single cell RNA sequencing (scRNA-seq) to analyze all cells from the ventricles.

2022-07-16 | GSE208162 | GEO

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