Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The Early Origins and Evolution of microRNAs and piRNAs in Animals

ABSTRACT: We explored the potential for small RNA biology in organisms that diverged basal to Bilateria, identifying both miRNAs and piRNAs in Nematsotella and Amphimedon

ORGANISM(S): Nematostella vectensis Monosiga brevicollis Amphimedon queenslandica Trichoplax adhaerens

PROVIDER: GSE12578 | GEO | 2008/10/01

SECONDARY ACCESSION(S): PRJNA112627

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

The Early Origins and Evolution of microRNAs and piRNAs in Animals

Project description:We explored the potential for small RNA biology in organisms that diverged basal to Bilateria, identifying both miRNAs and piRNAs in Nematsotella and Amphimedon Employ HTS of endogenous small RNAs

2008-09-30 | E-GEOD-12578 | biostudies-arrayexpress

The Early Origins and Evolution of microRNAs and piRNAs in Animals

Project description:The Early Origins and Evolution of microRNAs and piRNAs in Animals

| PRJNA112627 | ENA

Early origins and evolution of microRNAs and Piwi-interacting RNAs in animals.

Project description: Not available

| S-EPMC3837422 | biostudies-literature

Function, targets and evolution of Caenorhabditis elegans piRNAs (mRNA)

Project description:piRNAs are required to maintain germline integrity and fertility but their mechanism of action is poorly understood. Here we demonstrate that C. elegans piRNAs silence transcripts in trans through imperfectly complementary sites. We find that target silencing is independent of Piwi endonuclease activity or “slicing”. Instead, we show that piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene, pseudogene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes but not pseudogenes or transposons. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNAi in C. elegans.

2012-06-18 | GSE37432 | GEO

Function, targets and evolution of Caenorhabditis elegans piRNAs

Project description:This SuperSeries is composed of the following subset Series: GSE28617: Function, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq) GSE37432: Function, targets and evolution of Caenorhabditis elegans piRNAs (mRNA) Refer to individual Series

2012-06-17 | E-GEOD-37433 | biostudies-arrayexpress

Function, targets and evolution of Caenorhabditis elegans piRNAs (mRNA)

2012-06-17 | E-GEOD-37432 | biostudies-arrayexpress

Function, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq)

2012-06-18 | GSE28617 | GEO

Function, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq)

2012-06-18 | E-GEOD-28617 | biostudies-arrayexpress

Maternally inherited piRNAs silence transposons during Drosophila embryogenesis

Project description:The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of maternally deposited piRNAs and its associated factors during embryogenesis. Here, we probe the spatio-temporal expression patterns of several piRNA pathway components during early stages of development. Amongst those, factors required for transcriptional gene silencing (TGS) showed ubiquitous abundance in somatic and pole cells throughout the first half of embryogenesis. We further analysed the transcriptomes of various embryo stages and correlated these with the presence of selected chromatin marks. We found that a number of transposon families show bursts of transcription during early embryonic stages. Transposons heavily targeted by maternally deposited piRNAs accumulated repressive chromatin marks following their spike in expression. Furthermore, depletion of maternally deposited Piwi protein in early embryos resulted in increased expression of transposons targeted by inherited piRNAs and was accompanied by a strong loss of repressive chromatin marks at coding sequences. Overall, our data suggests a pivotal role for the piRNA pathway in transposon defence during Drosophila embryogenesis in somatic cells.

2021-07-26 | PXD022409 | Pride

Somatic piRNAs in veterbrate kidney

Project description:Somatic piRNAs in veterbrate kidney

| PRJNA753512 | ENA